Prolonged treatment with N-acetylcystine delays liver recovery from acetaminophen hepatotoxicity 英文参考文献.docVIP

Available online /content/13/2/R55 Research Open Access Vol 13 No 2 Prolonged treatment with N-acetylcystine delays liver recovery from acetaminophen hepatotoxicity Runkuan Yang1, Keita Miki1, Xin He2, Meaghan E Killeen1 and Mitchell P Fink1 1Department of Critical Care Medicine, University of Pittsburgh Medical School, 3550 Terrace Street, Pittsburgh, PA 15261, USA 2Department of Pathology, University of Pittsburgh Medical School, 3550 Terrace Street, Pittsburgh, PA 15261, USA Corresponding author: Runkuan Yang, Yangr@ Received: 3 Jan 2009 Revisions requested: 21 Feb 2009 Revisions received: 23 Mar 2009 Accepted: 9 Apr 2009 Published: 9 Apr 2009 Critical Care 2009, 13:R55 (doi:10.1186/cc7782) This article is online at: /content/13/2/R55 ? 2009 Yang et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License /licenses/by/2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Introduction Acetaminophen (APAP) toxicity is the most common cause of acute liver failure in the US and Europe. Massive hepatocyte necrosis is the predominant feature of APAP-induced acute liver injury (ALI). Liver regeneration is a vital process for survival after a toxic insult, it occurs at a relative late time point after the injurious phase. Currently, N-acetylcysteine hours of APAP challenge, the mice were given 100 mg/kg NAC dissolved in 0.6 mL saline, or saline treatment every 12 hours for a total of 72 hours. Results Seventy-two hours after APAP challenge, compared (NAC), a glutathione precursor, is the antidote for with saline treatment, NAC treatment significantly increased transaminase/aspartate aminotransferase), induced evident hepatocyte vacuolation in the periportal area and delayed liver regeneration seen in histopathology. This detrimental effect was asso