Proteasome and NF-κB Inhibiting Phaeophytins from the Green Alga Cladophora fascicularis 英文参考文献.docVIP

Molecules 2007, 12, 582-592 molecules ISSN 1420-3049 Full Paper Proteasome and NF-κB Inhibiting Phaeophytins from the Green Alga Cladophora fascicularis Xinping Huang 1,2, Min Li 1,*, Bo Xu 1, Xiaobin Zhu 2, Zhiwei Deng 3 and Wenhan Lin 1,? 1 State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100083, P.R. China, Tel: (+86) 10 Fax: (+86) 102 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China 3 Analytic and Testing Center, Beijing Normal University, Beijing 100073, P.R. China * Authors to whom correspondence may be addressed; E-mails: limin@; whlin@ Received: 18 February 2007; in revised form: 18 March 2007 / Accepted: 21 March 2007 / Published: 21 March 2007 Abstract: Chemical examination of the green alga Cladophora fascicularis resulted in the isolation and characterization of a new porphyrin derivative, porphyrinolactone (1), along with five known phaeophytins 2-6 and fourteen sterols and cycloartanes. The structure of 1 was determined on the basis of spectroscopic analyses and by comparison of its NMR data with those of known phaeophytins. Compounds 1-6 displayed moderate inhibition of tumor necrosis factor alpha (TNF-α) induced nuclear factor-κB (NF-κB) activation, while 2 and 4 displayed potential inhibitory activity toward proteasome chymotripsin-like activation. The primary structure-activity relationship was also discussed. Keywords: Green alga, Cladophora fascicularis, porphyrinolactone, NF-κB, proteasome Introduction The genus Cladophora (Cladophoraceae) is a group of filamentous green algae that grows widely in eutrophic wastewater and marine intertidal zones. Previous chemical investigations indicated that  Molecules 2007, 12 583 Cladophora mainly containing sterol derivatives [1-3]. Terpenes [4] from C. vagabunda in Black Sea and a brominated diphenyl ether from C. fascicularis [5] w