Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.) 英文参考文献.docVIP

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Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.) 英文参考文献.doc

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Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.) 英文参考文献

Guoetal.BMCPlantBiology2012,12:147 /1471-2229/12/147 RESEARCH ARTICLE OpenAccess Proteomecharacterizationofdevelopinggrainsin breadwheatcultivars(TriticumaestivumL.) GuangfangGuo?,DongwenLv?,XingYan?,SaminathanSubburaj,PeiGe,XiaohuiLi,YingkaoHu andYuemingYan* Abstract Background:Theanalysesofproteinsynthesis,accumulationandregulationduringgraindevelopmentinwheat aremorecomplexbecauseofitslargergenomesizecomparedtomodelplantssuchasArabidopsisandrice.In thisstudy,grainsfromtwowheatcultivarsJimai20andZhoumai16withdifferentglutenqualitypropertieswere harvestedatfivedevelopmentstages,andwereusedtodisplayedvariableexpressionpatternsofgrainproteins. Results:ProteomecharacterizationduringgraindevelopmentinChinesebreadwheatcultivarsJimai20and Zhoumai16withdifferentqualitypropertieswasinvestigatedby2-DEandtandemMALDI-TOF/TOF-MS. Identificationof117differentiallyaccumulatedproteinspotsrepresenting82uniqueproteinsandfivemain expressionpatternsenabledachronologicaldescriptionofwheatgrainformation.Significantproteomeexpression differencesbetweenthetwocultivarswerefound;theseincluded14proteinspotsthataccumulatedinboth cultivarsbutwithdifferentpatternsand27cultivar-differentspots.Amongthecultivar-differentproteinspots, 14accumulatedinhigherabundanceinJimai20thaninZhoumai16,andincludedNAD-dependentisocitrate dehydrogenase,triticinprecursor,LMW-sgluteninsubunitandreplicationfactorC-likeprotein.Theseproteinsare likelytobeassociatedwithsuperiorglutenquality.Inaddition,someproteinssuchasclassIIchitinaseand peroxidase1withisoformsindevelopinggrainswereshowntobephosphorylatedbyPro-QDiamondstainingand phosphorproteinsiteprediction.Phosphorylationcouldhaveimportantrolesinwheatgraindevelopment.qRT-PCR analysisdemonstratedthattranscriptionalandtranslationalexpressionpatternsofmanygenesweresignificantly different. Conclusions:Wheatgrainproteinsdisplayedvariableexpressionpatternsatdifferentdevelopmentalstagesanda considerablenumberofproteinspotsshoweddifferentialaccumulationbetweentwocultiva