Proteomics of Trypanosoma evansi Infection in Rodents 英文参考文献.docVIP

ProteomicsofTrypanosomaevansiInfectioninRodents NainitaRoy1.,RishiKumarNageshan1.,RaniPallavi1,HarshiniChakravarthy1,SyamaChandran1, RajenderKumar2,AshokKumarGupta2,RajKumarSingh2,SureshChandraYadav2,UtpalTatu1* 1DepartmentofBiochemistry,IndianInstituteofScience,Bangalore,India,2NationalResearchCentreonEquines,Hisar,India Abstract Background: Trypanosoma evansi infections, commonly called ‘surra’, cause significant economic losses to livestock industry. While this infection is mainly restricted to large animals such as camels, donkeys and equines, recent reports indicatetheirabilitytoinfecthumans.TherearenoWorldAnimalHealthOrganization(WAHO)prescribeddiagnostictests or vaccines available against this disease and the available drugs show significant toxicity. There is an urgent need to developimprovedmethodsofdiagnosisandcontrolmeasuresforthisdisease.UnlikeitsrelatedhumanparasitesT.brucei and T. cruzi whose genomes have been fully sequenced T. evansi genome sequence remains unavailable and very little efforts are being made to develop improved methods of prevention, diagnosis and treatment. With a view to identify potential diagnostic markers and drug targets we have studied the clinical proteome of T. evansi infection using mass spectrometry(MS). Methodology/PrincipalFindings:Usingshot-gunproteomicapproachinvolvingnano-lcQuadrupoleTimeOfFlight(QTOF) mass spectrometry we have identified over 160 proteins expressed by T. evansi in mice infected with camel isolate. Homology driven searches for protein identification from MS/MS data led to most of the matches arising from related Trypanosoma species. Proteins identified belonged to various functional categories including metabolic enzymes; DNA metabolism;transcription;translationaswellascell-cellcommunicationandsignaltransduction.TCAcycleenzymeswere strikinglymissing,possiblysuggestingtheirlowabundances.Theclinicalproteomerevealedthepresenceofknownand potentialdrugtargetssuchasoligopeptidases,kinases,cysteineproteases