Pseudomonas aeruginosa AES-1 Exhibits Increased Virulence Gene Expression during Chronic Infection of Cystic Fibrosis Lung 英文参考文献.docVIP

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Pseudomonas aeruginosa AES-1 Exhibits Increased Virulence Gene Expression during Chronic Infection of Cystic Fibrosis Lung 英文参考文献.doc

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Pseudomonas aeruginosa AES-1 Exhibits Increased Virulence Gene Expression during Chronic Infection of Cystic Fibrosis Lung 英文参考文献

PseudomonasaeruginosaAES-1ExhibitsIncreased VirulenceGeneExpressionduringChronicInfectionof CysticFibrosisLung SharnaNaughton1,DaneParker2,TorstenSeemann3,TorstenThomas4,LynneTurnbull2,5 ,Barbara Rose1,PeterBye6,7,StuartCordwell8,CynthiaWhitchurch2,5,JimManos1* 1DepartmentofInfectiousDiseasesandImmunology,UniversityofSydney,Sydney,Australia,2DepartmentofMicrobiology,MonashUniversity,Melbourne,Australia, 3Victorian Bioinformatics Consortium, Monash University, Melbourne, Australia, 4School of Biotechnology and Biomolecular Sciences and Centre for Marine Bio- Innovation,UniversityofNewSouthWales,Sydney,Australia,5Theithreeinstitute,UniversityofTechnology,Sydney,Australia,6DepartmentofRespiratoryMedicine, RoyalPrinceAlfredHospital,Sydney,Australia,7SydneyMedicalSchool,UniversityofSydney,Sydney,Australia,8SchoolofMolecularBiosciences,UniversityofSydney, Sydney,Australia Abstract Pseudomonasaeruginosa,theleadingcauseofmorbidityandmortalityinpeoplewithcysticfibrosis(CF),adaptsforsurvival intheCFlungthroughbothmutationandgeneexpressionchanges.FrequentclonalstrainssuchastheAustralianEpidemic Strain-1 (AES-1), have increased ability to establish infection in the CF lung and to superimpose and replace infrequent clonal strains. Little is known about the factors underpinning these properties. Analysis has been hampered by lack of expression array templates containing CF-strain specific genes. We sequenced the genome of an acute infection AES-1 isolatefromaCFinfant(AES-1R)andconstructedanon-redundantmicro-array(PANarray)comprisingAES-1Randseven othersequencedP.aeruginosagenomes.TheunclosedAES-1Rgenomecomprised6.254Mbpandcontained6957putative genes,including338notfoundintheothersevengenomes.ThePANarraycontained12,543geneprobespots;comprising 12,147P.aeruginosageneprobes,326quality-controlprobesand70probesfornon-P.aeruginosagenes,includingphage and plant genes. We grew AES-1R and its isogenic pair AES-1M, taken from the same patient 10.5 years later and not eradicatedintheinter