Pub1p C-Terminal RRM Domain Interacts with Tif4631p through a Conserved Region Neighbouring the Pab1p Binding Site 英文参考文献.docVIP

下载本文档

11
0
约7.58万字
约 17页
2017-05-13 发布于上海
举报
版权申诉

Pub1p C-Terminal RRM Domain Interacts with Tif4631p through a Conserved Region Neighbouring the Pab1p Binding Site 英文参考文献.doc

1、本文档共17页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Pub1p C-Terminal RRM Domain Interacts with Tif4631p through a Conserved Region Neighbouring the Pab1p Binding Site 英文参考文献

Pub1pC-TerminalRRMDomainInteractswithTif4631p throughaConservedRegionNeighbouringthePab1p BindingSite ClaraM.Santiveri.¤,YasminaMirassou.,PalmaRico-Lastres,SantiagoMart?′nez-Lumbreras.,Jose′ ManuelPe′rez-Can?adillas* DepartmentofBiologicalPhysicalChemistry,InstitutodeQu?′mica-F?′sica‘‘Rocasolano’’,CSIC,Madrid,Spain Abstract Pub1p, a highly abundant poly(A)+ mRNA binding protein in Saccharomyces cerevisiae, influences the stability and translationalcontrolofmanycellulartranscripts,particularlyundersometypesofenvironmentalstresses.Wehavestudied thestructure,RNAandproteinrecognitionmodesofdifferentPub1pconstructsbyNMRspectroscopy.Thestructureofthe C-terminal RRMdomain (RRM3) shows anon-canonical N-terminal helix that packs against the canonical RRM fold in an originalfashion.ThisstructuraltraitisconservedinPub1pmetazoanhomologues,theTIA-1family,defininganewclassof RRM-typedomainsthatweproposetonameTRRM(TIA-1C-terminaldomain-likeRRM).Pub1pTRRMandtheN-terminal RRM1-RRM2tandembindRNAwithhighselectivityforU-richsequences,withTRRMshowingadditionalpreferenceforUA- richones.RNA-mediatedchemicalshiftchangesmaptob-sheetandproteinloopsinthethreeRRMs.Additionally,NMR titrationandbiochemicalinvitrocross-linkingexperimentsdeterminedthatPub1pTRRMinteractsspecificallywiththeN- terminal region (1–402) of yeast eIF4G1 (Tif4631p), very likely through the conserved Box1, a short sequence motif neighbouringthePab1pbindingsiteinTif4631p.TheinteractioninvolvesconservedresiduesofPub1pTRRM,whichdefine aproteininterfacethatmirrorsthePab1p-Tif4631pbindingmode.NeitherproteinnorRNArecognitioninvolvesthenovel N-terminalhelix,whosefunctionalroleremainsunclear.Byintegratingthesenewresultswiththecurrentknowledgeabout Pub1p,weproposeddifferentmechanismsofPub1precruitmenttothemRNPsandPub1p-mediatedmRNAstabilizationin whichthePub1p/Tif4631pinteractionwouldplayanimportantrole. Citation: Santiveri CM, Mirassou Y, Rico-Lastres P, Mart?′nez-Lumbreras S, Pe′rez-Can?adillas JM (2011) Pub1p C-Terminal RRM Do