Pyrite-induced hydroxyl radical formation and its effect on nucleic acids 英文参考文献.docVIP

Geochemical Transactions BioMedCentral Research article Open Access Pyrite-induced hydroxyl radical formation and its effect on nucleic acids Corey A Cohn*1,5, Steffen Mueller2, Eckard Wimmer2, Nicole Leifer3, Steven Greenbaum3, Daniel R Strongin4,5 and Martin AA Schoonen1,5 Address: 1Department of Geosciences, Stony Brook University, Stony Brook, NY 11794, USA, 2Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794-2100, USA, 3Department of Physics, Hunter College, New York, NY 10021, USA, 4Department of Chemistry, Beury Hall 201, 1901 N. 13th Street, Temple University, Philadelphia, PA 19122, USA and 5Center for Environmental Molecular Science, Stony Brook University, Stony Brook, Stony Brook, NY 11794-2100, USA Email: Corey A Cohn* - corey.cohn@; Steffen Mueller - smueller@; Eckard Wimmer - ewimmer@; Nicole Leifer - nicole.d.leifer@; Steven Greenbaum - steve.greenbaum@; Daniel R Strongin - dstrongi@; Martin AA Schoonen - martin.schoonen@ * Corresponding author Published: 04 April 2006 Received: 30 December 2005 Accepted: 04 April 2006 Geochemical Transactions 2006, 7:3 doi:10.1186/1467-4866-7-3 This article is available from: /content/7/1/3 ? 2006 Cohn et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Pyrite, the most abundant metal sulphide on Earth, is known to spontaneously form hydrogen peroxide when exposed to water. In this study the hypothesis that pyrite-induced hydrogen peroxide is transformed to hydroxyl radicals is tested. Results: Using a combination of electron spin resonance (ESR) spin-trapping techniques and scavenging reactions involving nucleic acids, the formation of hydroxyl radicals in pyrite/aqueous suspens