Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection 英文参考文献.docVIP

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Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection 英文参考文献.doc

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Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection 英文参考文献

QuantitativeProteomicsofSpodopterafrugiperdaCells duringGrowthandBaculovirusInfection NunoCarinhas1,AaronMarkRobitaille2,SuzetteMoes2,ManuelJose′ TeixeiraCarrondo1,3,PaulJenoe2, RuiOliveira4,PaulaMarquesAlves1* 1Instituto de Tecnologia Qu?′mica e Biolo′gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnolo′gica, Oeiras, Portugal, 2Department of Biochemistry,BiozentrumoftheUniversityofBasel,Basel,Switzerland,3DepartamentodeQu?′mica,FaculdadedeCie?nciaseTecnologia,UniversidadeNovadeLisboa, Caparica,Portugal,4REQUIMTE,SystemsBiologyandEngineeringGroup(SBE),DepartamentodeQu?′mica,FaculdadedeCie?ncias eTecnologia,UniversidadeNovade Lisboa,Caparica,Portugal Abstract Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperdaisnotsequencedandthevirus-hostmolecularinterplayissparselyknown.Herein,wedescribetheapplicationof stableisotopelabelingbyaminoacidsincellculture(SILAC)toobtainthefirstcomparativeproteomequantitationof S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism,endoplasmicreticulumandoxidativestress,yetnotinvestigatedinthescopeofbaculovirusinfection.Further, thereducedexpressionofkeyviral-encodedproteinsearlyintheinfectioncycleissuggestedtoberelatedwithdecreased viralreplicationathighcelldensityculture.Thesefindingshaveimplicationsforvirologicalresearchandimprovementof baculovirus-basedbioprocesses. Citation: Carinhas N, Robitaille AM, Moes S, Carrondo MJT, Jenoe P, et al. (2011) Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and BaculovirusInfection.PLoSONE6(10):e26444.doi:10.1371/journal.pone.0026444 Editor:PaulDi