Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay 英文参考文献.docVIP

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Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay 英文参考文献.doc

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Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay 英文参考文献

Lisowskaetal.GenomeIntegrity2010,1:8 /content/1/1/8 GENOME INTEGRITY RESEARCH OpenAccess Radiation-inducedDNAdamageandrepairin humangδandabT-lymphocytesanalysed bythealkalinecometassay HalinaLisowska1,MartaDeperas-Kaminska1,2,SiamakHaghdoost3,IngelaParmryd4,AndrzejWojcik1,3* Abstract Ithasbeenshownbyanumberofauthorsthattheradiosensitivityofperipheralbloodmononuclearcells(PBMC) ishigherincancerpatientscomparedtohealthydonors,whichisinterpretedasasignofgenomicinstability. PBMCarecomposedofdifferentcellsubpopulationswhicharedifferentlyradiosensitiveandthedifference betweencancerpatientsandhealthydonorscouldalsobeduetodifferentcompositionoftheirPBMCpools. Gamma-deltaT-lymphocytesplayanimportantroleinimmunosurveillanceandarepromisingcellsforimmuno- therapy.Theirabundanceisfrequentlyreducedincancerpatientssoshouldtheirsensitivitytoradiationbelower thanthatofotherT-lymphocytes,thiscould,atleastpartlyexplainthelowradiosensitivityofPBMCfromhealthy individualscomparedtocancerpatients.Thepresentinvestigationwascarriedouttotestthis.Usingthealkaline cometassayweanalysedthelevelofDNAdamageandrepairinisolatedgδT-lymphocytes,panT-lymphocytes andintotalPBMCexposedinvitrotogammaradiation.WefoundnodifferenceinthelevelofDNAdamageand thecapacityofDNArepairbetweentheTcellpopulations.Thisisthefirststudythataddressesthequestionof sensitivitytoradiationofgamma-deltaT-cells. Background attractedinterestiscomposedofT-lymphocyteswithgδ Humanperipheralbloodmononuclearcells(PBMC)are T-cell receptors (TCR). The reason for this is the dis- usedassurrogatetissuefortheassessmentofindividual covery that these cells contribute to immunity against sensitivity to ionising radiation [1]. Using the G2 cancer[19].Inperipheralbloodgδlymphocytesaccount chromosomal aberration assay it has been shown by a for less than 5% of total T-lymphocytes while their numberofauthorsthattheradiosensitivity ofPBMCis proportionintheintestinecanbemuchhigher[20]. higher in cancer patients compared to hea