Raman Microspectroscopy of Individual Algal Cells Sensing Unsaturation of Storage Lipids in vivo 英文参考文献.docVIP

Sensors 2010, 10, 8635-8651; doi:10.3390/s100908635 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo Ota Samek 1,*, Alexandr Joná? 1, Zdeněk Pilát 1, Pavel Zemánek 1, Ladislav Nedbal 2, Jan T?íska 2, Petr Kotas 2 and Martin Trtílek 3 1 Institute of Scientific Instruments of the AS CR, v.v.i., Academy of Sciences of the Czech Republic, Kralovopolska 147, 61264 Brno, Czech Republic; E-Mails: sasa@isibrno.cz (A.J.); pilat@isibrno.cz (Z.P.); pavlik@isibrno.cz (P.Z.) 2 Institute of Systems Biology and Ecology of the AS CR, v.v.i., Academy of Sciences of the Czech Republic, Zámek 136, 37333 Nové Hrady, Czech Republic; E-Mails: nedbal@greentech.cz (L.N.); triska@usbe.cas.cz (J.T.); kotas@usbe.cas.cz (P.K.) 3 Photon Systems Instruments, Drásov 470, 664 24 Drásov, Czech Republic; E-Mail: martin@psi.cz * Author to whom correspondence should be addressed; E-Mail: osamek@isibrno.cz; Tel.: +420-541-514-127; Fax: +420-541-514-402. Received: 30 July 2010; in revised form: 25 August 2010 / Accepted: 14 September 2010 / Published: 17 September 2010 Abstract: Algae are becoming a strategic source of fuels, food, feedstocks, and biologically active compounds. This potential has stimulated the development of innovative analytical methods focused on these microorganisms. Algal lipids are among the most promising potential products for fuels as well as for nutrition. The crucial parameter characterizing the algal lipids is the degree of unsaturation of the constituent fatty acids quantified by the iodine value. Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells. The Raman spectra were collected from three selected algal species immobilized in a