Reactive Oxygen Species-Induced Impairment of Endothelium-Dependent Relaxations in Rat Aortic Rings Protection by Methanolic Extracts of Phoebe grandis 英文参考文献.docVIP

Molecules 2011, 16, 2990-3000; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Reactive Oxygen Species-Induced Impairment of Endothelium- Dependent Relaxations in Rat Aortic Rings: Protection by Methanolic Extracts of Phoebe grandis Lau Yeh-Siang 1, Gopal Subramaniam 1, A. Hamid A. Hadi 2, Dharmani Murugan 1 and Mohd Rais Mustafa 1,* 1 Centre of Natural Products and Drug Discovery (CENAR), Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mails: lauyehsiang@ (L.Y.-S.); kadagam@ (G.S.); dharmani79@.my (D.M.) 2 Centre of Natural Products and Drug Discovery (CENAR), Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mail: ahamid@.my * Author to whom correspondence should be addressed; E-Mail: rais@.my; Fax: 603 Received: 10 February 2011; in revised form: 15 March 2011 / Accepted: 1 April 2011 / Published: 6 April 2011 Abstract: Generation of reactive oxygen species plays a pivotal role in the development of cardiovascular diseases. The present study describes the effects of the methanolic extract of Phoebe grandis (MPG) stem bark on reactive oxygen species-induced endothelial dysfunction in vitro. Endothelium-dependent (acetylcholine, ACh) and -independent relaxation (sodium nitroprusside, SNP) was investigated from isolated rat aorta of Sprague- Dawley (SD) in the presence of the β-NADH (enzymatic superoxide inducer) and MPG extract. Superoxide anion production in aortic vessels was measured by lucigen chemiluminesence. Thirty minutes incubation of the rat aorta in vitro with β-NADH increased superoxide radical production and significantly inhibited ACh-induced relaxations. Pretreatment with MPG (0.5, 5 and 50 μg/mL) restored the ACh-induced relaxations (Rmax: 92.29% ± 2.93, 91.02% ± 4.54 and 88.31 ± 2.36, re