Resolution of volatile fuel compound profiles from Ascocoryne sarcoides a comparison by proton transfer reaction-mass spectrometry and solid phase microextraction gas chromatography-mass spectrometry 英文参考文献.docVIP

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Resolution of volatile fuel compound profiles from Ascocoryne sarcoides a comparison by proton transfer reaction-mass spectrometry and solid phase microextraction gas chromatography-mass spectrometry 英文参考文献.doc

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Resolution of volatile fuel compound profiles from Ascocoryne sarcoides a comparison by proton transfer reaction-mass spectrometry and solid phase microextraction gas chromatography-mass spectrometry 英文参考文献

Malletteetal.AMBExpress2012,2:23 /content/2/1/23 ORIGINAL ARTICLE OpenAccess Resolutionofvolatilefuelcompoundprofiles fromAscocorynesarcoides:acomparisonby protontransferreaction-massspectrometryand solidphasemicroextractiongaschromatography- massspectrometry NatashaDMallette1,2,WBerkKnighton3,GaryAStrobel4,RossPCarlson1,2 andBrentMPeyton1,2* Abstract VolatilehydrocarbonproductionbyAscocorynesacroideswasstudiedoveritsgrowthcycle.Gas-phasecompounds weremeasuredcontinuouslywithaprotontransferreaction-massspectrometry(PTR-MS)andatdistincttime pointswithgaschromatography-massspectrometry(GC-MS)usingheadspacesolidphasemicroextraction(SPME). ThePTR-MSionsignalpermittedtemporalresolutionofthevolatileproductionwhiletheSPMEresultsrevealed distinctcompoundidentities.ThequantitativePTR-MSresultsshowedthevolatileproductionwasdominatedby ethanolandacetaldehyde,whiletheconcentrationoftheremainderofvolatilesconsistentlyreached2,000ppbv. Themeasurementofalcoholsfromthefungalculturebythetwotechniquescorrelatedwell.Notablecompounds offuelinterestincludednonanal,1-octen-3-ol,1-butanol,3-methyl-andbenzaldehyde.Abioticcomparisonofthe twotechniquesdemonstratedSPMEfiberbiastowardhighermolecularweightcompounds,makingquantitative effortswithSPMEimpractical.Together,PTR-MSandSPMEGC-MSwereshownasvaluabletoolsforcharacterizing volatilefuelcompoundproductionfrommicrobiologicalsources. Keywords:Biofuel,Solidphasemicroextraction,Protontransferreaction-massspectrometry,Volatileorganiccom- pounds,Fungalhydrocarbons,Gaschromatography-massspectrometry Introduction heptane, 2-pentene, octane, 1-methyl-cyclohexene, 3,5- Ascocorynesarcoidesisanendophyticfungusrecentlyiso- octadiene,andcyclodecene[SolomonsandFryhle2002; latedfromNorthernPatagonia andhasthepotential to Griffin, Spakowicz, Gianoulis and Strobel 2010]. This produceapetroleum-likefuel(Mycodiesel?)directlyfrom interestingmetabolismrequiresfurtherworktocharacter- acellulosefermentationprocess[Strobel,Knighton,Kluck, iz