Rice Snl6, a Cinnamoyl-CoA Reductase-Like Gene Family Member, Is Required for NH1-Mediated Immunity to Xanthomonas oryzae pv. oryzae 英文参考文献.docVIP
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Rice Snl6, a Cinnamoyl-CoA Reductase-Like Gene Family Member, Is Required for NH1-Mediated Immunity to Xanthomonas oryzae pv. oryzae 英文参考文献
RiceSnl6,aCinnamoyl-CoAReductase-LikeGeneFamily
Member,IsRequiredforNH1-MediatedImmunityto
Xanthomonasoryzaepv.oryzae
RebeccaS.Bart1,2.,MawshengChern1,2.,MiguelE.Vega-Sa′nchez1,2,PatrickCanlas1,2
PamelaC.Ronald1,2
,
*
1DepartmentofPlantPathology,UniversityofCaliforniaDavis,Davis,California,UnitedStatesofAmerica,2JointBioenergyInstitute,Emeryville,California,UnitedStates
ofAmerica
Abstract
RiceNH1(NPR1homolog1)isakeymediatorofinnateimmunity.Inbothplantsandanimals,theinnateimmuneresponse
is often accompanied by rapid cell death at the site of pathogen infection. Over-expression of NH1 in rice results in
resistancetothebacterialpathogen,Xanthomonasoryzaepv.oryzae(Xoo),constitutiveexpressionofdefenserelatedgenes
andenhanced benzothiadiazole (BTH)- mediated cell death. Herewedescribe aforward geneticscreen that identified a
suppressorofNH1-mediatedlesionformationandresistance,snl6.Comparativegenomehybridizationandfinemapping
rapidlyidentifiedthegenomiclocationoftheSnl6gene.Snl6isamemberofthecinnamoyl-CoAreductase(CCR)-likegene
family. We show that Snl6 is required for NH1-mediated resistance to Xoo. Further, we show that Snl6 is required for
pathogenesis-relatedgeneexpression.IncontrasttopreviouslydescribedCCRfamilymembers,disruptionofSnl6doesnot
resultinanobviousmorphologicphenotype.Snl6mutantshavereducedlignincontentandincreasedsugarextractability,
animportanttraitfortheproductionofcellulosicbiofuels.TheseresultssuggesttheexistenceofaconservedgroupofCCR-
likegenesinvolvedinthedefenseresponse,andwiththepotentialtoalterlignincontentwithoutaffectingdevelopment.
Citation:BartRS,ChernM,Vega-Sa′nchezME,CanlasP,RonaldPC(2010)RiceSnl6,aCinnamoyl-CoAReductase-LikeGeneFamilyMember,IsRequiredforNH1-
MediatedImmunitytoXanthomonasoryzaepv.oryzae.PLoSGenet6(9):e1001123.doi:10.1371/journal.pgen.1001123
Editor:GregoryP.Copenhaver,TheUniversityofNorthCarolinaatChapelHill,UnitedStatesofAmerica
ReceivedFebruary19,2010;AcceptedAugust13,2010;PublishedSeptember16,2010
Copyright:?2010Barte
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