Rice Snl6, a Cinnamoyl-CoA Reductase-Like Gene Family Member, Is Required for NH1-Mediated Immunity to Xanthomonas oryzae pv. oryzae 英文参考文献.docVIP

Rice Snl6, a Cinnamoyl-CoA Reductase-Like Gene Family Member, Is Required for NH1-Mediated Immunity to Xanthomonas oryzae pv. oryzae 英文参考文献.doc

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Rice Snl6, a Cinnamoyl-CoA Reductase-Like Gene Family Member, Is Required for NH1-Mediated Immunity to Xanthomonas oryzae pv. oryzae 英文参考文献

RiceSnl6,aCinnamoyl-CoAReductase-LikeGeneFamily Member,IsRequiredforNH1-MediatedImmunityto Xanthomonasoryzaepv.oryzae RebeccaS.Bart1,2.,MawshengChern1,2.,MiguelE.Vega-Sa′nchez1,2,PatrickCanlas1,2 PamelaC.Ronald1,2 , * 1DepartmentofPlantPathology,UniversityofCaliforniaDavis,Davis,California,UnitedStatesofAmerica,2JointBioenergyInstitute,Emeryville,California,UnitedStates ofAmerica Abstract RiceNH1(NPR1homolog1)isakeymediatorofinnateimmunity.Inbothplantsandanimals,theinnateimmuneresponse is often accompanied by rapid cell death at the site of pathogen infection. Over-expression of NH1 in rice results in resistancetothebacterialpathogen,Xanthomonasoryzaepv.oryzae(Xoo),constitutiveexpressionofdefenserelatedgenes andenhanced benzothiadiazole (BTH)- mediated cell death. Herewedescribe aforward geneticscreen that identified a suppressorofNH1-mediatedlesionformationandresistance,snl6.Comparativegenomehybridizationandfinemapping rapidlyidentifiedthegenomiclocationoftheSnl6gene.Snl6isamemberofthecinnamoyl-CoAreductase(CCR)-likegene family. We show that Snl6 is required for NH1-mediated resistance to Xoo. Further, we show that Snl6 is required for pathogenesis-relatedgeneexpression.IncontrasttopreviouslydescribedCCRfamilymembers,disruptionofSnl6doesnot resultinanobviousmorphologicphenotype.Snl6mutantshavereducedlignincontentandincreasedsugarextractability, animportanttraitfortheproductionofcellulosicbiofuels.TheseresultssuggesttheexistenceofaconservedgroupofCCR- likegenesinvolvedinthedefenseresponse,andwiththepotentialtoalterlignincontentwithoutaffectingdevelopment. Citation:BartRS,ChernM,Vega-Sa′nchezME,CanlasP,RonaldPC(2010)RiceSnl6,aCinnamoyl-CoAReductase-LikeGeneFamilyMember,IsRequiredforNH1- MediatedImmunitytoXanthomonasoryzaepv.oryzae.PLoSGenet6(9):e1001123.doi:10.1371/journal.pgen.1001123 Editor:GregoryP.Copenhaver,TheUniversityofNorthCarolinaatChapelHill,UnitedStatesofAmerica ReceivedFebruary19,2010;AcceptedAugust13,2010;PublishedSeptember16,2010 Copyright:?2010Barte

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