Role of Mex67-Mtr2 in the Nuclear Export of 40S Pre-Ribosomes 英文参考文献.docVIP

RoleofMex67-Mtr2intheNuclearExportof40SPre- Ribosomes MariusB.Faza1,2.,YimingChang1.,LauraOcchipinti1,2,StefanKemmler1,VikramG.Panse1* 1 Institute of Biochemistry (IBC), ETH Zurich, Zurich, Switzerland,2 MLS Program, Life Science Zurich Graduate School, Zurich, Switzerland Abstract Nuclear export of mRNAs and pre-ribosomal subunits (pre40S and pre60S) is fundamental to all eukaryotes. While genetic approaches in budding yeast have identified bona fide export factors for mRNAs and pre60S subunits, little is known regarding nuclear export of pre40S subunits. The yeast heterodimeric transport receptor Mex67-Mtr2 (TAP-p15 in humans) binds mRNAs and pre60S subunits in the nucleus and facilitates their passage through the nuclear pore complex (NPC) into the cytoplasm by interacting with Phe-Gly (FG)-rich nucleoporins that line its transport channel. By exploiting a combination of genetic, cell-biological, and biochemical approaches, we uncovered an unanticipated role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes. We show that recruitment of Mex67-Mtr2 to pre40S subunits requires loops emanating from its NTF2-like domains and that the C-terminal FG-rich nucleoporin interacting UBA-like domain within Mex67 contributes to the transport of pre40S subunits to the cytoplasm. Remarkably, the same loops also recruit Mex67-Mtr2 to pre60S subunits and to the Nup84 complex, the respective interactions crucial for nuclear export of pre60S subunits and mRNAs. Thus Mex67-Mtr2 is a unique transport receptor that employs a common interaction surface to participate in the nuclear export of both pre-ribosomal subunits and mRNAs. Mex67-Mtr2 could engage a regulatory crosstalk among the three major export pathways for optimal cellular growth and proliferation. Citation: Faza MB, Chang Y, Occhipinti L, Kemmler S, Panse VG (2012) Role of Mex67-Mtr2 in the Nuclear Exp