胃癌腹腔种植转移模型小肠电生理变化及哇巴因干预效应.pdfVIP

第47 卷第2 期 哈尔滨医科大学学报 VoI. 47 ,No.2 112 2013 年4 月 JOURNAL OF HARBIN MEDICAL UNIVERSπY Apr. , 2013 胃癌腹腔种植转移模型小肠电生理变化 及哇巴因干预效应 赵宝山，宋印利，樊宁，许圣洁，邵畅，贺岩，金晓明* (哈尔滨医科大学病理学教研室，黑龙江哈尔滨 15∞81) [摘要] 曲曲串 模拟胃癌腹腔种植转移模型，探讨胃肠恶性肿瘤晚期常常出现肠蠕动减慢，似肠梗阻 症状，与小肠电生理的变化有无关系，应用哇巴因(ouabain) 能否改善这种变化。方旅 41 只裸鼠分为4 组，空白组5 只，培养液组4 只，SGC-7901 胃癌细胞系建立模型32 只(模型组 8 只，哇巴因处理 24 只作 为用药组)。应用 RM6240 B/C 多道生物信号采集与处理系统进行数据采集。锥.. ①模型鼠的电生 理显示，其波幅和频率比正常裸鼠的低，且差异有统计学意义(P 0. 05) 。②应用哇巴因进行干预后， 模型鼠的披幅有明显升高(PO.OI) ， 频率有改变，但无统计学意义。③应用肿瘤细胞培养液的裸鼠电 生理与正常比较显示，披幅明显升高(P 0.01) ，频率无统计学意义。份份 肿瘤在腹腔的种植转移能 够降低小肠的电活动，应用哇巴因可以改善小肠的放电强度。 [关键词] 胃癌腹腔种植模型;肠蠕动;小肠电生理;哇巴因;干预 [中固分类号]R-332 ;R735. 2 [文献标识码]A [文章编号] lC削- 1905 (2013 )02 - 0112 - 05 Electrophysiological changes and ouabain intervention effects of small intes- tine in gastric cancer peritoneal met:astasis model ZHAO Bao-shan , SONG Yin-li , FAN Ning , XU Sheng-jie , SHAO Chang , HE Yan , JIN Xiao- mmg (Depαrtment 0/ Pαthology ， lJ，αrbin Medical University , Harbin 150081 , China) Abstract: Objective To investigate whether the reduced gastrointestinal motility (similar to the symtom of intestinal obstruction) appearing at advanced gastrointestinal cancer was related to the intestine electrical physiological changes and whether ouabain could improve the changes by establishing the mouse model of gastric cancer peritoneal metastasis. Methods Forty-one BALB/cASlac-nu mice were divided into four grot,I ps: 5 for the control group , 4 for culture so- lution group , 32 for the peritoneal metastasis group (8 for model control group and 24 for oua- bain group). The intestinal myoelectrical activity was recorded using a multiplechannel record- er (model RM6240B) for 20 min. Results 四le amplitude and frequency of the peritoneal metastasi