Role of Structure and Glycosylation of Adsorbed Protein Films in Biolubrication 英文参考文献.docVIP

RoleofStructureandGlycosylationofAdsorbedProtein FilmsinBiolubrication DeepakH.Veeregowda1,HenkJ.Busscher1,ArjanVissink2,Derk-JanJager2,PrashantK.Sharma1*, HennyC.vanderMei1 1Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands, 2Department of Oral MaxillofacialSurgery,UniversityMedicalCenterGroningenandUniversityofGroningen,Groningen,TheNetherlands Abstract Waterformsthebasisoflubricationinthehumanbody,butisunabletoprovidesufficientlubricationwithoutadditives.The importanceofbiolubricationbecomesevidentuponaginganddisease,particularlyunderconditionsthataffectsecretionor compositionofbodyfluids.Insufficientbiolubrication,mayimpedeproperspeech,masticationandswallowing,underlie excessive friction and wear of articulating cartilage surfaces in hips and knees, cause vaginal dryness, and result in dry, irritated eyes. Currently, our understanding of biolubrication is insufficient to design effective therapeutics to restore biolubrication. Aim of this study was to establish the role of structure and glycosylation of adsorbed protein films in biolubrication,takingtheoralcavityasamodelandmakinguseofitsdynamicswithdailyperturbationsduetodifferent glandular secretions, speech, drinking and eating, and tooth brushing. Using different surface analytical techniques (a quartz crystal microbalance with dissipation monitoring, colloidal probe atomic force microscopy, contact angle measurementsandX-rayphoto-electronspectroscopy),wedemonstratedthatadsorbedsalivaryconditioningfilmsinvitro aremorelubriciouswhentheirhydrophilicityanddegreeofglycosylationincrease,meanwhiledecreasingtheirstructural softness.High-molecular-weight,glycosylatedproteinsadsorbinginloopsandtrains,aredescribedasnecessaryscaffolds impeding removal of water during loading of articulating surfaces. Comparing in vitro and in vivo water contact angles measuredintra-orally,thesefindingswereextrapolatedtotheinvivosituation.Accordingly,lubrica