Selective Histonedeacetylase Inhibitor M344 Intervenes in HIV-1 Latency through Increasing Histone Acetylation and Activation of NF-kappaB 英文参考文献.docVIP

下载本文档

10
0
约6.75万字
约 16页
2017-05-13 发布于上海
举报
版权申诉

Selective Histonedeacetylase Inhibitor M344 Intervenes in HIV-1 Latency through Increasing Histone Acetylation and Activation of NF-kappaB 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Selective Histonedeacetylase Inhibitor M344 Intervenes in HIV-1 Latency through Increasing Histone Acetylation and Activation of NF-kappaB 英文参考文献

SelectiveHistonedeacetylaseInhibitorM344Intervenes inHIV-1LatencythroughIncreasingHistoneAcetylation andActivationofNF-kappaB HaoYing.,YuhaoZhang.,XinZhou.,XiyingQu,PengfeiWang,SijieLiu,DaruLu,HuanzhangZhu* StateKeyLaboratoryofGeneticEngineering,InstituteofGenetics,SchoolofLifeSciences,FudanUniversity,Shanghai,China Abstract Background: Histone deacetylase (HDAC) inhibitors present an exciting new approach to activate HIV production from latentlyinfectedcellstopotentiallyenhanceeliminationofthesecellsandachieveacure.M344,anovelHDACinhibitor, showsrobustactivityinavarietyofcancercellsandrelativelylowtoxicitycomparedtotrichostatinA(TSA).However,littleis knownabouttheeffectsandactionmechanismofM344ininducingHIVexpressioninlatentlyinfectedcells. Methodology/Principal Findings: Using the Jurkat T cell model of HIV latency, we demonstrate that M344 effectively reactivatesHIV-1geneexpressioninlatentlyinfectedcells.Moreover,M344-mediatedactivationofthelatentHIVLTRcanbe stronglyinhibitedbyaNF-kBinhibitoraspirin.WefurthershowthatM344actsbyincreasingtheacetylationofhistoneH3 andhistoneH4atthenucleosome1(nuc-1)siteoftheHIV-1longterminalrepeat(LTR)andbyinducingNF-kBp65nuclear translocationanddirectRelADNAbindingatthenuc-1regionoftheHIV-1LTR.WealsofoundthatM344synergizedwith prostratintoactivatetheHIV-1LTRpromoterinlatentlyinfectedcells. Conclusions/Significance:TheseresultssuggestthepotentialofM344inanti-latencytherapiesandanimportantrolefor histonemodificationsandNF-kBtranscriptionfactorsinregulatingHIV-1LTRgeneexpression. Citation: Ying H, Zhang Y, Zhou X, Qu X, Wang P, et al. (2012) Selective Histonedeacetylase Inhibitor M344 Intervenes in HIV-1 Latency through Increasing HistoneAcetylationandActivationofNF-kappaB.PLoSONE7(11):e48832.doi:10.1371/journal.pone.0048832 Editor:FatahKashanchi,GeorgeMasonUniversity,UnitedStatesofAmerica ReceivedMarch21,2012;AcceptedOctober5,2012;PublishedNovember15,2012 Copyright:?2012Yingetal.Thisisanopen-accessarticledistributedunderthetermsoft