Sensing the Structural Differences in Cellulose from Apple and Bacterial Cell Wall Materials by Raman and FT-IR Spectroscopy 英文参考文献.docVIP
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Sensors 2011, 11, 5543-5560; doi:10.3390/s110605543
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Sensing the Structural Differences in Cellulose from Apple and
Bacterial Cell Wall Materials by Raman and FT-IR
Spectroscopy
Monika Szymańska-Chargot *, Justyna Cybulska and Artur Zdunek
Institute of Agrophysics, Polish Academy of Science, Do?wiadczalna 4, 20-290 Lublin, Poland;
E-Mails: j.cybulska@ipan.lublin.pl (J.C.); a.zdunek@ipan.lublin.pl (A.Z.)
* Author to whom correspondence should be addressed; E-Mail: m.szymanska@iapan.lublin.pl;
Tel.: +4-881-744-5061; Fax: +4-881-744-5067.
Received: 9 March 2011; in revised form: 3 April 2011 / Accepted: 7 April 2011 /
Published: 25 May 2011
Abstract: Raman and Fourier Transform Infrared (FT-IR) spectroscopy was used for
assessment of structural differences of celluloses of various origins. Investigated celluloses
were: bacterial celluloses cultured in presence of pectin and/or xyloglucan, as well as
commercial celluloses and cellulose extracted from apple parenchyma. FT-IR spectra were
used to estimate of the Iβ content, whereas Raman spectra were used to evaluate the degree
of crystallinity of the cellulose. The crystallinity index (XCRAMAN%) varied from ?25% for
apple cellulose to 53% for microcrystalline commercial cellulose. Considering bacterial
cellulose, addition of xyloglucan has an impact on the percentage content of cellulose Iβ.
However, addition of only xyloglucan or only pectins to pure bacterial cellulose both
resulted in a slight decrease of crystallinity. However, culturing bacterial cellulose in the
presence of mixtures of xyloglucan and pectins results in an increase of crystallinity. The
results confirmed that the higher degree of crystallinity, the broader the peak around
913 cm?1. Among all bacterial celluloses the bacterial cellulose cultured in presence of
xyloglucan and pectin (BCPX) has the most similar st
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