Serial Non-Invasive Monitoring of Renal Disease Following Immune-Mediated Injury Using Near-Infrared Optical Imaging 英文参考文献.docVIP

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Serial Non-Invasive Monitoring of Renal Disease Following Immune-Mediated Injury Using Near-Infrared Optical Imaging 英文参考文献.doc

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Serial Non-Invasive Monitoring of Renal Disease Following Immune-Mediated Injury Using Near-Infrared Optical Imaging 英文参考文献

SerialNon-InvasiveMonitoringofRenalDisease FollowingImmune-MediatedInjuryUsingNear-Infrared OpticalImaging YongDu1,ShionAn1,LiLiu2,LiLi1,XinJ.Zhou1,RalphP.Mason2*,ChandraMohan1* 1Internal Medicine/Rheumatology, University of Texas, Southwestern Medical Center, Dallas, Texas, United States of America, 2Radiology, University of Texas, SouthwesternMedicalCenter,Dallas,Texas,UnitedStatesofAmerica Abstract Background:Non-invasivemonitoringofdiseaseprogressioninkidneydiseaseisstillamajorchallengeinclinicalpractice. Invivonear-infrared(NIR)imagingprovidesanewtoolforstudyingdiseasemechanismsandnon-invasivemonitoringof diseasedevelopment,evenindeeporgans.TheLI-CORIRDyeH800CWRGDopticalprobe(RGDprobe)isaNIRfluorophore, thatcantargetintegrinalphavbeta3(avb3)intissues. Objective:Thisstudyaimstomonitorrenaldiseaseprogressioninananti-glomerularbasementmembrane(GBM)nephritis mousemodel. Methods:Anti-GBMnephritiswasinducedin129x1/svJmicebyanti-GBMserumchallenge.Theexpressionofintegrinavb3 in the diseased kidney was examined by immunohistochemistry and quantitative polymerase chain reaction. The RGD probe and control fluorophores, the 800CW dye, and the BSA-conjugated 800CW dye, were administered into anti-GBM nephritic mice. LI-COR PearlH Impulse imaging system was used for in vivo imaging; while ex vivo organ imaging was acquiredusingtheMaestroTM imagingsystem. Results:Kidneytissuefromanti-GBMnephriticmiceshowedhigherlevelsofintegrinavb3expressionatboththeprotein andthemRNAlevelcomparedtonormalmice.TheRGDprobeallowedinvivorenalimagingandthefluorescentsignal couldbespecificallycapturedinthediseasedkidneysupto14days,reflectinglongitudinalchangesinrenalfunction. Conclusion:Theinfrared RGDmolecular probethattracks integrinexpressioncanbe successfullyused tomonitor renal diseaseprogressionfollowingimmune-mediatednephritis. Citation:DuY,AnS,LiuL,LiL,ZhouXJ,etal.(2012)SerialNon-InvasiveMonitoringofRenalDiseaseFollowingImmune-MediatedInjuryUsingNear-Infrared OpticalImaging.PLoSONE7(9