Separation, Characterization and Dose-Effect Relationship of the PPARγ-Activating Bio-Active Constituents in the Chinese Herb Formulation ‘San-Ao Decoction’ 英文参考文献.docVIP

Molecules 2009, 14, 3942-3951; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Separation, Characterization and Dose-Effect Relationship of the PPARγ-Activating Bio-Active Constituents in the Chinese Herb Formulation ‘San-Ao Decoction’ Ling Zhou 1, Yu-Ping Tang 1,*, Lu Gao 2, Xin-Sheng Fan 1,*, Chun-Mei Liu 1 and De-Kang Wu 1 1 Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China 2 Nanjing Medical University, Nanjing 210029, China * Authors to whom correspondence should be addressed; E-Mails: yupingtang@ (Y-P.T.); ltcmf@ (X-S.F.). Received: 14 August 2009; in revised form: 9 September 2009 / Accepted: 29 September 2009 / Published: 9 October 2009 Abstract: San-ao decoction (SAD), comprising Herba Ephedrae, Radix et Rhizoma Glycyrrhizae and Seneb Armeniacae Amarum, is one of the most popular traditional Chinese medicine (TCM) formulae for asthma. Peroxisome proliferator-activated receptors (PPARs) are key regulators of lipid and glucose metabolism and have become important therapeutic targets for various deseases, PPARγ activation might exhibit anti-inflammatory properties in different chronic inflammatory processes. The EtOAc fraction of SAD showed a significant effect on PPARγ activation. A simple and rapid method has been established for separation and characterization of the main compounds in the PPARγ- activating fraction of SAD by ultra-fast HPLC coupled with quadropole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). A total of 10 compounds were identified in the activating fraction of SAD, including amygdalin (1), liquiritin (2), 6′-acetyliquiritin (3), liquiritigenin (4), isoliquiritigenin (5), formononetin (6), licoisoflavanone (7), glycycoumarin (8), glycyrol (9) and quercetin (10). The results also characterized formononetin