Sequence Analysis of pKF3-70 in Klebsiella pneumoniae Probable Origin from R100-Like Plasmid of Escherichia coli 英文参考文献.docVIP

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Sequence Analysis of pKF3-70 in Klebsiella pneumoniae Probable Origin from R100-Like Plasmid of Escherichia coli 英文参考文献.doc

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Sequence Analysis of pKF3-70 in Klebsiella pneumoniae Probable Origin from R100-Like Plasmid of Escherichia coli 英文参考文献

SequenceAnalysisofpKF3-70inKlebsiellapneumoniae: ProbableOriginfromR100-LikePlasmidofEscherichia coli HuiguangYi1,2.,YaliXi1.,JingLiu1,JunrongWang1,JinyuWu1,TengXu1,WeiChen1,BiaobangChen1, MeiliLin1,HuanWang1,MingmingZhou1,JinsongLi1,ZuyuanXu1,ShouguangJin3,QiyuBao1* 1InstituteofBiomedicalInformatics/ZhejiangProvincialKeyLaboratoryofMedicalGenetics,WenzhouMedicalCollege,Wenzhou,China,2T-LifeResearchCenter,Fudan University,Shanghai,China,3DepartmentofMolecularGeneticsandMicrobiology,UniversityofFlorida,Gainesville,Florida,UnitedStatesofAmerica Abstract Background:Klebsiellapneumoniaeisaclinicallysignificantspeciesofbacteriumwhichcausesavarietyofdiseases.Clinical treatmentofthisbacterialinfectionisgreatlyhinderedbytheemergenceofmultidrug-resistantstrains.Theresistanceis largely due to the acquisition of plasmids carrying drug-resistant as well as pathogenic genes, and its conjugal transfer facilitatesthespreadofresistantphenotypes. Methodology/PrincipalFindings:The70,057bpplasmidpKF3-70,commonlyfoundinKlebsiellapneumoniae,iscomposed of five main functional modules, including regions involved in replication, partition, conjugation, transfer leading, and variableregions.ThisplasmidismoresimilartoseveralEscherichiacoliplasmidsthananypreviouslyreportedK.pneumoniae plasmids and pKF3-70 like plasmids share a common and conserved backbone sequence. The replication system of the pKF3-70is100%identicaltothatofRepFIIplasmidR100fromE.coli.Abeta-lactamasegenectx-m-14withitssurrounding insertion elements (ISEcp1, truncated IS903 and a 20bp inverted repeat sequence) may compose an active transposon whichisdirectlyborderedbytwoputativetargetrepeats‘‘ATTAC.’’ Conclusions/Significance:TheK.pneumoniaeplasmidpKF3-70carriesanextended-spectrumbeta-lactamasegene,ctx-m- 14.Theconjugativecharacteristicmakesitawidespreadplasmidamonggeneticallyrelevantgenerawhichposessignificant threattopublichealth. Citation:YiH,XiY,LiuJ,WangJ,WuJ,etal.(2010)SequenceAnalysisofpKF3-70inKlebsiellapneumoniae:P