Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene 英文参考文献.docVIP

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Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene 英文参考文献.doc

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Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene 英文参考文献

Acta Veterinaria Scandinavica BioMedCentral Research Open Access Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene Azusa Takaesu1, Kiyotaka Watanabe1, Shinji Takai2, Yukako Sasaki2 and Koichi Orino*1 Address: 1Laboratories of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan and 2Animal Hygiene, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan Email: Azusa Takaesu - azu147@; Kiyotaka Watanabe - watanebe@vmas.ktiasato-u.ac.jp; Shinji Takai - takai@vmas.kitasato- u.ac.jp; Yukako Sasaki - yukako@vmas.kitasato-u.ac.jp; Koichi Orino* - orino@vmas.kitasato-u.ac.jp * Corresponding author Published: 27 October 2008 Received: 12 June 2008 Accepted: 27 October 2008 Acta Veterinaria Scandinavica 2008, 50:42 doi:10.1186/1751-0147-50-42 This article is available from: /content/50/1/42 ? 2008 Takaesu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Iron-storage protein, ferritin plays a central role in iron metabolism. Ferritin has dual function to store iron and segregate iron for protection of iron-catalyzed reactive oxygen species. Tissue ferritin is composed of two kinds of subunits (H: heavy chain or heart-type subunit; L: light chain or liver-type subunit). Ferritin gene expression is controlled at translational level in iron-dependent manner or at transcriptional level in iron-independent manner. However, sequencing analysis of marine mammalian ferritin subunits has not yet been performed fully. The purpose of this study is to reveal cDNA-derived amino acid sequences of cetacean ferritin H and L subunits, and de