Sequence Diversity within the Capsular Genes of Streptococcus pneumoniae Serogroup 6 and 19 英文参考文献.docVIP

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Sequence Diversity within the Capsular Genes of Streptococcus pneumoniae Serogroup 6 and 19 英文参考文献.doc

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Sequence Diversity within the Capsular Genes of Streptococcus pneumoniae Serogroup 6 and 19 英文参考文献

SequenceDiversitywithintheCapsularGenesof StreptococcuspneumoniaeSerogroup6and19 KarinElberse1*,SandraWitteveen1,HanvanderHeide1,IngridvandePol1,CorrieSchot1,Arievander Ende2,GuyBerbers1,LeoSchouls1 1Laboratory for Infectious Diseases and Perinatal Screening, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands, 2The NetherlandsReferenceLaboratoryforBacterialMeningitisandDepartmentofMedicalMicrobiology,AcademicMedicalCenter,Amsterdam,TheNetherlands Abstract The main virulence factor of Streptococcus pneumoniae is the capsule. The polysaccharides comprising this capsule are encodedbyapproximately15genesanddifferencesinthesegenesresultindifferentserotypes.Theaimofthisstudywas toinvestigatethesequencediversityofthecapsulargenesofserotypes6A,6B,6C,19Aand19Fandtoexploreapossible effectofvaccinationonvariationanddistributionoftheseserotypesintheNetherlands.Thecompletecapsulargenelocus wassequencedfor25serogroup6andfor20serogroup19isolates.Ifoneormoregenesvariedin10ormorebasepairs fromthereferencesequence,itwasdesignatedasacapsularsubtype.Allele-specificPCRsandspecificgenesequencingof highly variable capsular genes were performed on 184 serogroup 6 and 195 serogroup 19 isolates to identify capsular subtypes.Thisrevealedthepresenceof6,3andasinglecapsularsubtypewithinserotypes6A,6Band6C,respectively.The serotype19Aand19Fisolatescomprised3and4capsularsubtypes,respectively.Forserogroup6,thegeneticbackground, as determined by multi locus sequence typing (MLST) and multiple- locus variable number of tandem repeat analysis (MLVA),seemedtobecloselyrelatedtothecapsularsubtypes,butthiswaslesspronouncedforserogroup19isolates.The dataalsosuggestshiftsintheoccurrenceofcapsularsubtypeswithinserotype6Aand19Aafterintroductionofthe7-valent pneumococcalvaccine.Theshiftswithinthesenon-vaccineserotypesmightindicatethatthesecapsularsubtypesarefilling thenicheofthevaccineserotypes.Inconclusion,thereisconsiderableDNAsequencevariationofthecapsulargeneswith