SGNP An Essential Stress GranuleNucleolar Protein Potentially Involved in 5.8s rRNA ProcessingTransport 英文参考文献.docVIP

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SGNP An Essential Stress GranuleNucleolar Protein Potentially Involved in 5.8s rRNA ProcessingTransport 英文参考文献.doc

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SGNP An Essential Stress GranuleNucleolar Protein Potentially Involved in 5.8s rRNA ProcessingTransport 英文参考文献

SGNP:AnEssentialStressGranule/NucleolarProtein PotentiallyInvolvedin5.8srRNAProcessing/Transport Chun-HongZhu,JinyongKim,JerryW.Shay,WoodringE.Wright* DepartmentofCellBiology,UniversityofTexasSouthwesternMedicalCenter,Dallas,Texas,UnitedStatesofAmerica Abstract Background: Stress Granules (SG) are sites of accumulation of stalled initiation complexes that are induced following a varietyofcellularinsults.Inageneticscreenforfactorsinvolvedinprotectinghumanmyoblastsfromacuteoxidativestress, weidentifiedageneencodingaproteinwedesignateSGNP(StressGranuleandNucleolarProtein). Methodology/Principal Findings: A gene-trap insertional mutagenesis screen produced one insertion that conferred resistance to sodium arsenite. RT-PCR/39 RACE was used to identify the endogenous gene expressed as a GFP-fusion transcript.SGNPislocalizedinboththecytoplasmandnucleolusanddefinesanon-nucleolarcompartmentcontaining5.8S rRNA, a component of the 60S ribosomal subunit. Under oxidative stress, SGNP nucleolar localization decreases and it rapidlyco-localizeswithstressgranules.ThedecreaseinnucleolarSGNPfollowingoxidativestresswasaccompaniedbya large increase in nucleolar 5.8S rRNA. Knockdown of SGNP with shRNA increased global mRNA translation but induced growtharrestandcelldeath. Conclusions: These results suggest that SGNP is an essential gene that may be involved in ribosomal biogenesis and translationalcontrolinresponsetooxidativestress. Citation: Zhu C-H, Kim J, Shay JW, Wright WE (2008) SGNP: An Essential Stress Granule/Nucleolar Protein Potentially Involved in 5.8s rRNA Processing/ Transport.PLoSONE3(11):e3716.doi:10.1371/journal.pone.0003716 Editor:ThomasPreiss,VictorChangCardiacResearchInstitute,Australia ReceivedSeptember23,2008;AcceptedOctober17,2008;PublishedNovember13,2008 Copyright:?2008Zhuetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandso