Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae) proteomic and metabolic responses at early stage 英文参考文献.docVIP

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Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae) proteomic and metabolic responses at early stage 英文参考文献.doc

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Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae) proteomic and metabolic responses at early stage 英文参考文献

Palama et al. BMC Plant Biology 2010, 10:82 /1471-2229/10/82 RESEARCH ARTICLE Open Access Shoot differentiation from protocorm callus Research article cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage Tony L Palama1,2, Patrice Menard1, Isabelle Fock1, Young H Choi2, Emmanuel Bourdon3, Joyce Govinden-Soulange1,4, Muriel Bahut5, Bertrand Payet6, Robert Verpoorte2 and Hippolyte Kodja*1 Abstract Background: Vanilla planifolia is an important Orchid commercially cultivated for the production of natural vanilla flavour. Vanilla plants are conventionally propagated by stem cuttings and thus causing injury to the mother plants. Regeneration and in vitro mass multiplication are proposed as an alternative to minimize damage to mother plants. Because mass production of V. planifolia through indirect shoot differentiation from callus culture is rare and may be a successful use of in vitro techniques for producing somaclonal variants, we have established a novel protocol for the regeneration of vanilla plants and investigated the initial biochemical and molecular mechanisms that trigger shoot organogenesis from embryogenic/organogenic callus. Results: For embryogenic callus induction, seeds obtained from 7-month-old green pods of V. planifolia were inoculated on MS basal medium (BM) containing TDZ (0.5 mg l-1). Germination of unorganized mass callus such as protocorm -like structure (PLS) arising from each seed has been observed. The primary embryogenic calli have been formed after transferring on BM containing IAA (0.5 mg l-1) and TDZ (0.5 mg l-1). These calli were maintained by subculturing on BM containing IAA (0.5 mg l-1) and TDZ (0.3 mg l-1) during 6 months and formed embryogenic/ organogenic calli. Histological analysis showed that shoot organogenesis was induced between 15 and 20 days after embryogenic/organogenic calli were transferred onto MS basal medium with NAA (0.5 mg l-1). By associating proteomics an