SNP high-throughput screening in grapevine using the SNPlex？ genotyping system 英文参考文献.docVIP

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SNP high-throughput screening in grapevine using the SNPlex？ genotyping system 英文参考文献.doc

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SNP high-throughput screening in grapevine using the SNPlex？ genotyping system 英文参考文献

BMC Plant Biology BioMedCentral Methodology article Open Access SNP high-throughput screening in grapevine using the SNPlex? genotyping system Massimo Pindo*1, Silvia Vezzulli1, Giuseppina Coppola1, Dustin A Cartwright2, Andrey Zharkikh2, Riccardo Velasco1 and Michela Troggio1 Address: 1IASMA Research Center, Via E. Mach, 1 – I 38010 – San Michele allAdige, Italy and 2Myriad Genetics Inc, Wakara Way, 320 – UT 84108 – Salt Lake City, USA Email: Massimo Pindo* - massimo.pindo@iasma.it; Silvia Vezzulli - silvia.vezzulli@iasma.it; Giuseppina Coppola - giuseppina.coppola@iasma.it; Dustin A Cartwright - dustin.cartwright@; Andrey Zharkikh - zharkikh@; Riccardo Velasco - riccardo.velasco@iasma.it; Michela Troggio - michela.troggio@iasma.it * Corresponding author Published: 28 January 2008 Received: 20 September 2007 Accepted: 28 January 2008 BMC Plant Biology 2008, 8:12 doi:10.1186/1471-2229-8-12 This article is available from: /1471-2229/8/12 ? 2008 Pindo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Until recently, only a small number of low- and mid-throughput methods have been used for single nucleotide polymorphism (SNP) discovery and genotyping in grapevine (Vitis vinifera L.). However, following completion of the sequence of the highly heterozygous genome of Pinot Noir, it has been possible to identify millions of electronic SNPs (eSNPs) thus providing a valuable source for high-throughput genotyping methods. Results: Herein we report the first application of the SNPlex? genotyping system in grapevine aiming at the anchoring of an eukaryotic genome. This approach combines robust SNP detection with automated assay readout and data analysis. 813 candidate eSNPs were develop