SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis 英文参考文献.docVIP

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SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis 英文参考文献.doc

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SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis 英文参考文献

Available online /content/9/5/R107 Research article Open Access Vol 9 No 5 SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis Simon R Tew1, Peter D Clegg1,2, Christopher J Brew1, Colette M Redmond2 and Timothy E Hardingham1 1UK Centre for Tissue Engineering, Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK 2Faculty of Veterinary Sciences, University of Liverpool, Leahurst, Neston, CH64 7TE, UK Corresponding author: Timothy E Hardingham, timothy.e.hardingham@manchester.ac.uk Received: 10 Aug 2007 Revisions requested: 30 Aug 2007 Revisions received: 26 Sep 2007 Accepted: 12 Oct 2007 Published: 12 Oct 2007 Arthritis Research Therapy 2007, 9:R107 (doi:10.1186/ar2311) This article is online at: /content/9/5/R107 ? 2007 Tew et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract The transcription factor SOX9 is important in maintaining the chondrocyte phenotype. To identify novel genes regulated by SOX9 we investigated changes in gene expression by microarray analysis following retroviral transduction with SOX9 of a human chondrocytic cell line (SW1353). From the results the expression of a group of genes (SRPX, S100A1, APOD, RGC32, CRTL1, MYBPH, CRLF1 and SPINT1) was evaluated further in human articular chondrocytes (HACs). First, the same genes were investigated in primary cultures of HACs following SOX9 transduction, and four were found to be similarly regulated (SRPX, APOD, CRTL1 and S100A1). Second, during dedifferentiation of HACs by passage in monolayer cell culture, during which the