Soybean GmPHD-Type Transcription Regulators Improve Stress Tolerance in Transgenic Arabidopsis Plants 英文参考文献.docVIP

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Soybean GmPHD-Type Transcription Regulators Improve Stress Tolerance in Transgenic Arabidopsis Plants 英文参考文献.doc

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Soybean GmPHD-Type Transcription Regulators Improve Stress Tolerance in Transgenic Arabidopsis Plants 英文参考文献

SoybeanGmPHD-TypeTranscriptionRegulatorsImprove StressToleranceinTransgenicArabidopsisPlants WeiWei1.,JianHuang1.,Yu-JunHao1,Hong-FengZou1,Hui-WenWang1,Jing-YunZhao2,Xue-YiLiu2, Wan-KeZhang1,BiaoMa1,Jin-SongZhang1*,Shou-YiChen1* 1PlantGeneResearchCenter,NationalKeyLaboratoryofPlantGenomics,InstituteofGeneticsandDevelopmentalBiology,ChineseAcademyofSciences,Beijing,China, 2Instituteofeconomiccrops,ShanxiAcademyofAgriculturalSciences,Fenyang,Shanxi,China Abstract Background:Soybean[Glycinemax(L.)Merr.]isoneofthemostimportantcropsforoilandproteinresource.Improvement ofstresstolerancewillbebeneficialforsoybeanseedproduction. Principal Findings: Six GmPHD genes encoding Alfin1-type PHD finger protein were identified and their expressions differentially responded to drought, salt, cold and ABA treatments. The six GmPHDs were nuclear proteins and showed abilitytobindthecis-element‘‘GTGGAG’’.TheN-terminaldomainofGmPHDplayedamajorroleinDNAbinding.Usinga protoplastassaysystem,wefindthatGmPHD1toGmPHD5hadtranscriptionalsuppressionactivitywhereasGmPHD6did nothave.Inyeastassay,theGmPHD6canformhomodimerandheterodimerwiththeotherGmPHDsexceptGmPHD2.The N-terminalplusthevariableregionsbutnotthePHD-fingerisrequiredforthedimerization.TransgenicArabidopsisplants overexpressing the GmPHD2 showed salt tolerance when compared with the wild type plants. This tolerance was likely achievedbydiminishingtheoxidativestressthroughregulationofdownstreamgenes. Significance: These results provide important clues for soybean stress tolerance through manipulation of PHD-type transcriptionregulator. Citation:WeiW,HuangJ,HaoY-J,ZouH-F,WangH-W,etal.(2009)SoybeanGmPHD-TypeTranscriptionRegulatorsImproveStressToleranceinTransgenic ArabidopsisPlants.PLoSONE4(9):e7209.doi:10.1371/journal.pone.0007209 Editor:HanyA.El-Shemy,CairoUniversity,Egypt ReceivedApril24,2009;AcceptedSeptember7,2009;PublishedSeptember30,2009 Copyright:?2009Weietal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAt