Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts 英文参考文献.docVIP

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Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts 英文参考文献.doc

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Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts 英文参考文献

Open Access M d 2006HahneetVoal.lumeetho7, Issue 8, Article R77 Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts Florian Hahne*, Dorit Arlt*, Mamatha Sauermann*, Meher Majety*, Annemarie Poustka*, Stefan Wiemann* and Wolfgang Huber? Addresses: *Division of Molecular Genome Analysis, German Cancer Research Center, INF 580, 69120 Heidelberg, Germany. ?EMBL - European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge CB10 1SD, UK. Correspondence: Florian Hahne. Email: f.hahne@dkfz.de Published: 17 August 2006 Received: 18 May 2006 Revised: 7 July 2006 Accepted: 17 August 2006 Genome Biology 2006, 7:R77 (doi:10.1186/gb-2006-7-8-r77) The electronic version of this article is the complete one and can be found online at /2006/7/8/R77 ? 2006 Hahne et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. SoftwarepA softforwarhigh-throughpute tool for the anacytometrylysis of higassaysh-throughput cell-based assays is presented./p Abstract Highthroughput cell-based assays with flow cytometric readout provide a powerful technique for identifying components of biologic pathways and their interactors. Interpretation of these large datasets requires effective computational methods. We present a new approach that includes data pre-processing, visualization, quality assessment, and statistical inference. The software is freely available in the Bioconductor package prada. The method permits analysis of large screens to detect the effects of molecular interventions in cellular systems. Background nonsurvival. More detail can be seen from a quantitative var- iable such as the activity of a reporter gene measured on a flu- orescent plate r