Statistical tests for differential expression in cDNA microarray experiments 英文参考文献.docVIP

Review Statistical tests for differential expression in cDNA microarray experiments Xiangqin Cui and Gary A Churchill Address: The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA. Correspondence: Gary A Churchill. E-mail: garyc@ Published: 17 March 2003 Genome Biology 2003, 4:210 The electronic version of this article is the complete one and can be found online at /2003/4/4/210 ? 2003 BioMed Central Ltd Abstract Extracting biological information from microarray data requires appropriate statistical methods. The simplest statistical method for detecting differential expression is the t test, which can be used to compare two conditions when there is replication of samples. With more than two conditions, analysis of variance (ANOVA) can be used, and the mixed ANOVA model is a general and powerful approach for microarray experiments with multiple factors and/or several sources of variation. Gene-expression microarrays hold tremendous promise for revealing the patterns of coordinately regulated genes. Because of the large volume and intrinsic variation of the data obtained in each microarray experiment, statistical methods have been used as a way to systematically extract biological information and to assess the associated uncer- tainty. Here, we review some widely used methods for testing differential expression among conditions. For these purposes, we assume that the data to be used are of good quality and have been appropriately transformed (normalized) to ensure that experimentally introduced biases have been removed [1,2]. See Box 1 for a glossary of terms. For other aspects of microarray data analysis, please refer to recent reviews on experimental design [3,4] and cluster analysis [5]. conditions are compared directly, this implies that the true ratio between the expression of each gene in the two samples should be one. When samples