Tissue sampling methods and standards for vertebrate genomics 英文参考文献.docVIP

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Tissue sampling methods and standards for vertebrate genomics 英文参考文献.doc

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Tissue sampling methods and standards for vertebrate genomics 英文参考文献

Wongetal.GigaScience2012,1:8 /content/1/1/8 REVIEW OpenAccess Tissuesamplingmethodsandstandardsfor vertebrategenomics PamelaBYWong1,2,EdwardOWiley3,WarrenEJohnson4,OliverARyder5,StephenJO’Brien6,DavidHaussler7, Klaus-PeterKoepfli4,MarlysLHouck5,PolinaPerelman8,GabrielaMastromonaco9,AndrewCBentley3, ByrappaVenkatesh10,Ya-pingZhang12,13,RobertWMurphy1,2,12* andG10KCOS11 Abstract Therecentriseinspeedandefficiencyofnewsequencingtechnologieshavefacilitatedhigh-throughput sequencing,assemblyandanalysesofgenomes,advancingongoingeffortstoanalyzegeneticsequencesacross majorvertebrategroups.StandardizedproceduresinacquiringhighqualityDNAandRNAandestablishingcelllines fromtargetspecieswillfacilitatetheseinitiatives.Weprovidealegalandmethodologicalguideaccordingtofour standardsofacquiringandstoringtissuefortheGenome10KProjectandsimilarinitiativesasfollows:four-star (bankedtissue/cellcultures,RNAfrommultipletypesoftissuefortranscriptomes,andsufficientflash-frozentissue for1mgofDNA,allfromasingleindividual);three-star(RNAasaboveandfrozentissuefor1mgofDNA);two-star (frozentissueforatleast700μgofDNA);andone-star(ethanol-preservedtissuefor700μgofDNAorlessofmixed quality).Ataminimum,alltissuescollectedfortheGenome10Kandothergenomicprojectsshouldconsidereach species’naturalhistoryandfollowinstitutionalandlegalrequirements.Associateddocumentationshoulddetailas muchinformationaspossibleaboutprovenancetoensurerepresentativesamplingandsubsequentsequencing. Hopefully,theproceduresoutlinedherewillnotonlyencouragesuccessintheGenome10KProjectbutalsoinspire theadaptationofstandardsbyothergenomicprojects,includingthoseinvolvingotherbiota. Keywords:Genome10K,Sequencing,Vertebrates,Genomics,Tissuesampling,Tissuestorage,Cellline,Tissue culture,RNA,DNA Review ecological and environmental interactions [5-7]. In this Advancesinsequencing technology overthelastdecade regard, the “Genomics Era” [7] holds promise for new [1-3] have made it feasible to acquire a database for population-genomicap