TMA for all a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles 英文参考文献.docVIP

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TMA for all a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles 英文参考文献.doc

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TMA for all a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles 英文参考文献

Diagnostic Pathology BioMedCentral Methodology Open Access TMA for all: a new method for the construction of tissue microarrays without recipient paraffin block using custom-built needles Andréa Rodrigues Cordovil Pires*?1, Felipe da Matta Andreiuolo?2 and Simone Rabello de Souza?2 Address: 1Fonte Medicina Diagnóstica Ltda. and Fluminense Federal University, Rio de Janeiro, Niterói, Brazil and 2Fonte Medicina Diagnóstica Ltda., Rio de Janeiro, Niterói, Brazil Email: Andréa Rodrigues Cordovil Pires* - andreapires@.br; Felipe da Matta Andreiuolo - andreiuolo@; Simone Rabello de Souza - admin@.br * Corresponding author ?Equal contributors Published: 25 July 2006 Received: 16 July 2006 Accepted: 25 July 2006 Diagnostic Pathology 2006, 1:14 doi:10.1186/1746-1596-1-14 This article is available from: /content/1/1/14 ? 2006 Pires et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: TMAs are becoming a useful tool for research and quality control methods, mostly for immunohistochemistry and in situ hybridization. Methods: A new technique that allows building TMA blocks with more than 300 tissue cores without using a recipient paraffin block for the tissue cores and without using a commercial TMA builder instrument is described. This technique is based on the construction of TMA needles modifying conventional hypodermic needles to punch tissue cores from donor blocks, which are attached by double-side adhesive tape on a computer-generated paper grid used to align the cores on the block mould, which is filled with liquid paraffin. Results: More than two hundred TMA blocks were constructed using this method, utilized in immunohistochemistry and histochemistry as positive and n