Tomato root transcriptome response to a nitrogen-enriched soil patch 英文参考文献.docVIP

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Tomato root transcriptome response to a nitrogen-enriched soil patch 英文参考文献.doc

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Tomato root transcriptome response to a nitrogen-enriched soil patch 英文参考文献

Ruzicka et al. BMC Plant Biology 2010, 10:75 /1471-2229/10/75 RESEARCH ARTICLE Open Access Tomato root transcriptome response to a Research article nitrogen-enriched soil patch Daniel R Ruzicka1, Felipe H Barrios-Masias2, Natasha T Hausmann2, Louise E Jackson2 and Daniel P Schachtman*1 Abstract Background: Nitrogen (N), the primary limiting factor for plant growth and yield in agriculture, has a patchy distribution in soils due to fertilizer application or decomposing organic matter. Studies in solution culture over- simplify the complex soil environment where microbial competition and spatial and temporal heterogeneity challenge roots ability to acquire adequate amounts of nutrients required for plant growth. In this study, various ammonium treatments (as 15N) were applied to a discrete volume of soil containing tomato (Solanum lycopersicum) roots to simulate encounters with a localized enriched patch of soil. Transcriptome analysis was used to identify genes differentially expressed in roots 53 hrs after treatment. Results: The ammonium treatments resulted in significantly higher concentrations of both ammonium and nitrate in the patch soil. The plant roots and shoots exhibited increased levels of 15N over time, indicating a sustained response to the enriched environment. Root transcriptome analysis identified 585 genes differentially regulated 53 hrs after the treatments. Nitrogen metabolism and cell growth genes were induced by the high ammonium (65 μg NH4+-N g-1 soil), while stress response genes were repressed. The complex regulation of specific transporters following the ammonium pulse reflects a simultaneous and synergistic response to rapidly changing concentrations of both forms of inorganic N in the soil patch. Transcriptional analysis of the phosphate transporters demonstrates cross-talk between N and phosphate uptake pathways and suggests that roots increase phosphate uptake via the arbuscular mycorrhizal symbiosis in response to N. C