Toward a Continuous Intravascular Glucose Monitoring System 英文参考文献.docVIP

Sensors 2011, 11, 409-424; doi:10.3390/s110100409 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Toward a Continuous Intravascular Glucose Monitoring System Brooke Beier 1,*, Katherine Musick 1, Akira Matsumoto 2, Alyssa Panitch 1, Eric Nauman 1,3,4 and Pedro Irazoqui 1 1 The Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; E-Mails: kmmusick@ (K.M.); apanitch@ (A.P.); enauman@ (E.N.); pip@ (P.I.) 2 Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 Japan; E-Mail: amatsumoto@bmw.t.u-tokyo.ac.jp (A.M.) 3 Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907, USA 4 School of Mechanical Engineering, Purdue University, West Lafayette, IN 47907, USA * Author to whom correspondence should be addressed; E-Mail: bbeier@; Tel.: +1-810-571-2387; Fax: +1-765-494-1912. Received: 11 November 2010; in revised form: 25 December 2010 / Accepted: 26 December 2010 / Published: 31 December 2010 Abstract: Proof-of-concept studies that display the potential of using a glucose-sensitive hydrogel as a continuous glucose sensor are presented. The swelling ratio, porosity, and diffusivity of the hydrogel increased with glucose concentration. In glucose solutions of 50, 100, 200, and 300 mg/dL, the hydrogel swelling ratios were 4.9, 12.3, 15.9, and 21.7, respectively, and the swelling was reversible. The impedance across the hydrogel depended solely on the thickness and had an average increase of 47 ?/mm. The hydrogels exposed to a hyperglycemic solution were more porous than the hydrogels exposed to a normal glycemic solution. The diffusivity of 390 Da MW fluorescein isothiocyanate in hydrogels exposed to normal and hyperglycemic solutions was examined using fluorescence recovery after photobleaching and was found to be 9.3 × 10?14 and 41.4 × 10?14 m2/s, respectively, compared to 6.2 × 10?10 m2/s in glucose soluti