Toxin Detection by Surface Plasmon Resonance 英文参考文献.docVIP

Sensors 2009, 9, 1339-1354; doi:10.3390/s9031339 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Toxin Detection by Surface Plasmon Resonance Vesna Hodnik and Gregor Anderluh* University of Ljubljana, Biotechnical Faculty, Department of Biology, Ve?na pot 111, 1000 Ljubljana, Slovenia; E-Mail: vesna.hodnik@bf.uni-lj.si (V.H.) * Author to whom correspondence should be addressed; E-Mail: gregor.anderluh@bf.uni-lj.si; Tel.: +386-1-423-33-88; Fax: +386-1-257-33-90 Received: 3 November 2008; in revised form: 19 January 2009 / Accepted: 20 February 2009 / Published: 26 February 2009 Abstract: Significant efforts have been invested in the past years for the development of analytical methods for fast toxin detection in food and water. Immunochemical methods like ELISA, spectroscopy and chromatography are the most used in toxin detection. Different methods have been linked, e.g. liquid chromatography and mass spectrometry (LC-MS), in order to detect as low concentrations as possible. Surface plasmon resonance (SPR) is one of the new biophysical methods which enables rapid toxin detection. Moreover, this method was already included in portable sensors for on-site determinations. In this paper we describe some of the most common methods for toxin detection, with an emphasis on SPR. Keywords: Surface plasmon resonance, toxins, detection, mycotoxins, ricin, enterotoxin, aflatoxin, atrazine. 1. Introduction The great diversity of toxins represents a big challenge when effective detection is needed. Different chemical and physicochemical characteristics of the analytes lead to specific extraction, sample cleanup and detection. Detection methods are, therefore, specific for individual toxins or groups of similar toxins. Fast and accurate methods are needed in order to deal with the increasing number of toxins of interest. The analyses should avoid large costs and be