Towards accurate imputation of quantitative genetic interactions 英文参考文献.docVIP

Open Access M d 2009UlitskyetVoal.lumeetho10, Issue 12, Article R140 Towards accurate imputation of quantitative genetic interactions Igor Ulitsky*?, Nevan J Krogan? and Ron Shamir* Addresses: *Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv 69978, Israel. ?Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158, USA. ?Current address: Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA. Correspondence: Ron Shamir. Email: rshamir@tau.ac.il Published: 10 December 2009 Received: 1 September 2009 Revised: 8 November 2009 Accepted: 10 December 2009 Genome Biology 2009, 10:R140 (doi:10.1186/gb-2009-10-12-r140) The electronic version of this article is the complete one and can be found online at /2009/10/12/R140 ? 2009 Ulitsky et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited YeastchparAomgeneticneywcems ceeinteractionsthodrevisforaecalculating/it./p quantitative genetic interactions allows for the inference of 190,000 new genetic interactions in itSac- Abstract Recent technological breakthroughs have enabled high-throughput quantitative measurements of hundreds of thousands of genetic interactions among hundreds of genes in Saccharomyces cerevisiae. However, these assays often fail to measure the genetic interactions among up to 40% of the studied gene pairs. Here we present a novel method, which combines genetic interaction data together with diverse genomic data, to quantitatively impute these missing interactions. We also present data on almost 190,000 novel interactions. Background dSLAM (heterozygote diploid-based synthetic lethality analy- sis with microarrays) [7,8], have ma