Towards an integrated proteomic and glycomic approach to finding cancer biomarkers 英文参考文献.docVIP

Review Towards an integrated proteomic and glycomic approach to finding cancer biomarkers Allen D Taylor*, William S Hancock?, Marina Hincapie?, Naoyuki Taniguchi?§ and Samir M Hanash* Addresses: *Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA. ?Department of Chemistry and Chemical Biology, Barnett Institute, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA. ?Systems Glycobiology Group, Disease Glycomics Team, Advanced Science Institute, RIKEN, Japan. §Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka, Japan. Correspondence: Samir M Hanash. Email: shanash@ Published: 4 June 2009 Genome Medicine 2009, 1:57 (doi:10.1186/gm57) The electronic version of this article is the complete one and can be found online at /content/1/6/57 ? 2009 BioMed Central Ltd Abstract Advances in mass spectrometry have had a great impact on the field of proteomics. A major challenge of proteomic analysis has been the elucidation of glycan modifications of proteins in complex proteomes. Glycosylation is the most structurally elaborate and diverse type of protein post-translational modification and, because of this, proteomics and glycomics have largely developed independently. However, given that such a large proportion of proteins contain glycan modifications, and that these may be important for their function or may produce biologically relevant protein variation, a convergence of the fields of glycomics and proteomics would be highly desirable. Here we review the current status of glycoproteomic efforts, focusing on the identification of glycoproteins as cancer biomarkers. Introduction translational modification and has been shown to have The sequencing of the human genome and the spectacular advances in mass spectrometry (MS) have had a substantial impact on the field of proteomics. MS has evolved