Tol2 a versatile gene transfer vector in vertebrates 英文参考文献.docVIP

Review Tol2: a versatile gene transfer vector in vertebrates Koichi Kawakami Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, The Graduate University of Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan Correspondence: Koichi Kawakami. E-mail: kokawaka@lab.nig.ac.jp Published: 31 October 2007 Genome Biology 2007, 8(Suppl 1):S7 (doi:10.1186/gb-2007-8-S1-S7) The electronic version of this article is the complete one and can be found online at /2007/8/S1/S7 ? 2007 BioMed Central Ltd Abstract The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has 200 and 150 base pairs of DNA from the left and right ends of the Tol2 sequence, respectively. These sequences contain essential terminal inverted repeats and subterminal sequences. DNA inserts of fairly large sizes (as large as 11 kilobases) can be cloned between these sequences without reducing transpositional activity. The Tol2 transposon system has been shown to be active in all vertebrate cells tested thus far, including zebrafish, Xenopus, chicken, mouse, and human. In this review I describe and discuss how the Tol2 transposon is being applied to transgenic studies in these vertebrates, and possible future applications. Introduction The minimal Tol2 vector and its capacity The Tol2 element was identified from the genome of the medaka fish (Oryzias latipes), a small fresh water teleost. The sequence of Tol2 is similar to those of transposons belonging to the hAT family, namely hobo, Ac and Tam3 [1]. Although vertebrate genomes contain a large number of sequences related to DNA transposons, including those similar to the hAT family transposons, none of these has been shown to be naturally active, and