Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin 英文参考文献.docVIP

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Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin 英文参考文献.doc

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Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias (Streptophyta), with emphasis on the role of expansin 英文参考文献

Vannerumetal.BMCPlantBiology2011,11:128 /1471-2229/11/128 RESEARCH ARTICLE OpenAccess Transcriptionalanalysisofcellgrowthand morphogenesisintheunicellulargreenalga Micrasterias(Streptophyta),withemphasisonthe roleofexpansin KatrijnVannerum1,2,3,MarieJJHuysman1,2,3,RietDeRycke2,3,MarnikVuylsteke2,3,FrederikLeliaert4,JacobPollier2,3, UrsulaLütz-Meindl5,JeroenGillard1,2,3,LievenDeVeylder2,3,AlainGoossens2,3,DirkInzé2,3 andWimVyverman1* Abstract Background:Streptophytegreenalgaeshareseveralcharacteristicsofcellgrowthandcellwallformationwith theirrelatives,theembryophyticlandplants.ThemultilobedcellwallofMicrasteriasdenticulatathatrebuilds symmetricallyaftercelldivisionandconsistsofpectinandcellulose,makesthisunicellularstreptophytealgaan interestingmodelsystemtostudythemolecularcontrolsoncellshapeandcellwallformationingreenplants. Results:Genome-widetranscriptexpressionprofilingofsynchronouslygrowingcellsidentified107genesofwhich theexpressioncorrelatedwiththegrowthphase.Fourtranscriptsshowedhighsimilaritytoexpansinsthathadnot beenexaminedpreviouslyingreenalgae.Phylogeneticanalysissuggeststhatthesegenesaremostcloselyrelated totheplantEXPANSINAfamily,althoughtheirdomainorganizationisverydivergent.AGFP-taggedversionofthe expansin-resemblingproteinMdEXP2localizedtothecellwallandinGolgi-derivedvesicles.Overexpression phenotypesrangedfromlobeelongationtolossofgrowthpolarityandplanarity.Theseresultsindicatethat MdEXP2canalterthecellwallstructureand,thus,mighthaveafunctionrelatedtothatoflandplantexpansins duringcellmorphogenesis. Conclusions:OurstudydemonstratesthepotentialofM.denticulataasaunicellularmodelsystem,inwhichcell growthmechanismshavebeendiscoveredsimilartothoseinlandplants.Additionally,evidenceisprovidedthat theevolutionaryoriginsofmanycellwallcomponentsandregulatorygenesinembryophytesprecedethe colonizationofland. Background understand howthese mechanisms have evolved inthe Although the form and function of plant cells are