Transcriptional profiling of Medicago truncatula meristematic root cells 英文参考文献.docVIP

BMC Plant Biology BioMedCentral Research article Open Access Transcriptional profiling of Medicago truncatula meristematic root cells Peta Holmes1, Nicolas Goffard1,2, Georg F Weiller1, Barry G Rolfe1 and Nijat Imin*1 Address: 1ARC Centre of Excellence for Integrative Legume Research, Genomic Interactions Group, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia and 2Institut Louis Malardé, GP Box 30, 98713 Papeete Tahiti, French Polynesia Email: Peta Holmes - peta.holmes@.au; Nicolas Goffard - ngoffard@ilm.pf; Georg F Weiller - georg.weiller@.au; Barry G Rolfe - barry.rolfe@.au; Nijat Imin* - nijat.imin@.au * Corresponding author Published: 27 February 2008 Received: 15 June 2007 Accepted: 27 February 2008 BMC Plant Biology 2008, 8:21 doi:10.1186/1471-2229-8-21 This article is available from: /1471-2229/8/21 ? 2008 Holmes et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The root apical meristem of crop and model legume Medicago truncatula is a significantly different stem cell system to that of the widely studied model plant species Arabidopsis thaliana. In this study we used the Affymetrix Medicago GeneChip? to compare the transcriptomes of meristem and non-meristematic root to identify root meristem specific candidate genes. Results: Using mRNA from root meristem and non-meristem we were able to identify 324 and 363 transcripts differentially expressed from the two regions. With bioinformatics tools developed to functionally annotate the Medicago genome array we could identify significant changes in metabolism, signalling and the differentially expression of 55 transcription factors in meristematic and non-me