Tracking cells in Life Cell Imaging videos using topological alignments 英文参考文献.docVIP

Algorithms for Molecular Biology BioMedCentral Research Open Access Tracking cells in Life Cell Imaging videos using topological alignments Axel Mosig*1,2, Stefan J?ger1, Chaofeng Wang1, Sumit Nath3, Ilker Ersoy3, Kannap-pan Palaniappan3 and Su-Shing Chen1 Address: 1Department of Combinatorics and Geometry, CAS-MPG Partner Institute for Computational Biology, 200031 Shanghai, PR China, 2Max Planck Institute for Mathematics in the Sciences, 04103 Leipzig, Germany and 3Department of Computer Science, University of Missouri- Columbia, Columbia MO 65211, USA Email: Axel Mosig* - axel@; Stefan J?ger - jaeger@; Chaofeng Wang - wangcf@; Sumit Nath - naths@; Ilker Ersoy - ersoy@; Kannap-pan Palaniappan - palaniappank@; Su-Shing Chen - suchen@ * Corresponding author Published: 16 July 2009 Received: 4 December 2008 Accepted: 16 July 2009 Algorithms for Molecular Biology 2009, 4:10 doi:10.1186/1748-7188-4-10 This article is available from: /content/4/1/10 ? 2009 Mosig et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: With the increasing availability of live cell imaging technology, tracking cells and other moving objects in live cell videos has become a major challenge for bioimage informatics. An inherent problem for most cell tracking algorithms is over- or under-segmentation of cells – many algorithms tend to recognize one cell as several cells or vice versa. Results: We propose to approach this problem through so-called topological alignments, which we apply to address the problem of linking segmentations of two consecutive frames in the video sequence. Starting from the output of a conventional segmentation procedure, we align pairs of consecutive frames through assigning set