Transcriptome changes in grapevine (Vitis vinifera L.) cv. Malbec leaves induced by ultraviolet-B radiation 英文参考文献.docVIP
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Transcriptome changes in grapevine (Vitis vinifera L.) cv. Malbec leaves induced by ultraviolet-B radiation 英文参考文献
Pontinetal.BMCPlantBiology2010,10:224
/1471-2229/10/224
RESEARCH ARTICLE
OpenAccess
Transcriptomechangesingrapevine
(VitisviniferaL.)cv.Malbecleavesinducedby
ultraviolet-Bradiation
MarielaAPontin1,2,PatriciaNPiccoli1,RitaFrancisco3,RubenBottini1*,JoseMMartinez-Zapater4,5,
DiegoLijavetzky1,4
Abstract
Background:Ultraviolet-Bradiation(UV-B,280-315nm)isanaturalcomponentofsunlight,whichhasnumerous
regulatoryeffectsonplantphysiology.ThenatureoftheresponsetoUV-Bisdependentonfluencerate,dose,
durationandwavelengthoftheUV-Btreatment.Somereportshaveanalyzedthechangesingeneexpression
causedbyUV-Blightonseveralplantspeciesusingmicroarraytechnology.However,thereisnoinformationon
thetranscriptomeresponsetriggeredbyUV-Bingrapevine.Inthispaperweinvestigatethegeneexpression
responsesofleavesfrominvitroculturedVitisviniferacv.Malbecplantssubjectedtothesamedoseofbiologically
effectiveUV-Bradiation(4.75kJm-2 d-1)administeredattwodifferentfluencerates(16hat?8.25μWcm-2,4hat
?33μWcm-2)usinganewcustommadeGrapeGenAffymetrixGeneChip?.
Results:ThenumberofgenesmodulatedbyhighfluencerateUV-Bdoubledthenumberofgenesmodulatedby
lowfluenceUV-B.Theirfunctionalanalysesrevealedseveralfunctionalcategoriescommonlyregulatedbyboth
UV-BtreatmentsaswellascategoriesmorespecificallymodulateddependingonUV-Bfluencerate.General
protectiveresponses,namelytheinductionofpathwaysregulatingsynthesisofUV-Babsorbingcompoundssuch
asthePhenylpropanoidpathway,theinductionofdifferentantioxidantdefensesystemsandtheactivationof
pathwayscommonlyassociatedwithpathogendefenseandabioticstressresponsesseemtoplaycriticalrolesin
grapevineresponsesagainstUV-Bradiation.Furthermore,highfluencerateUV-Bseemedtospecificallymodulate
additionalpathwaysandprocessesinordertoprotectgrapevineplantletsagainstUV-B-inducedoxidativestress,
stopthecellcycleprogression,andcontrolproteindegradation.Ontheotherhand,lowfluencerateUV-B
regulatedtheexpressionofspecificresponsesinthemetabolismofauxinandabscisicacidaswellasinthe
modificationofcellwall
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