Transformation of Geniposide into Genipin by Immobilized β-Glucosidase in a Two-Phase Aqueous-Organic System 英文参考文献.docVIP
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Transformation of Geniposide into Genipin by Immobilized β-Glucosidase in a Two-Phase Aqueous-Organic System 英文参考文献
Molecules 2011, 16, 4295-4304; doi:10.3390/moleculeOPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
Transformation of Geniposide into Genipin by Immobilized
β-Glucosidase in a Two-Phase Aqueous-Organic System
Yi-Shun Yang 1,2, Tong Zhang 1,*, Shai-Cheng Yu 2, Yue Ding 1, Li-Ying Zhang 1, Chen Qiu 2
and Duo Jin 2
1
Experiment Center of Teaching Learning, Shanghai University of Traditional Chinese Medicine,
Shanghai 201203, China; E-Mail: becky105@163.com (Y.-S.Y.)
2
College of Chemical Engineering, East China University of Science and Technology, Shanghai
200237, China; E-Mail: scyu@ (S.-C.Y.)
* Author to whom correspondence should be addressed; E-Mail: zhangtdmj@;
Tel.: +86 21 5132 2318; Fax: +86 21 5132 2332.
Received: 15 February 2011; in revised form: 3 May 2011 / Accepted: 13 May 2011 /
Published: 24 May 2011
Abstract: Genipin is the bioactive compound of geniposide and a natural cross-linking
agent. In order to improve the preparation process of genipin, the hydrolysis of geniposide
to genipin by immobilized β-glucosidase in an aqueous-organic two-phase system was
studied. β-Glucosidase was immobilized by the crosslinking-embedding method using
sodium alginate as the carrier. The optimum reaction temperature, pH value and time were
55 °C, 4.5 and 2.5 h, respectively. To reduce genipin hydrolysis and byproduct production
the reaction was carried out in an aqueous-organic two-phase system comprising ethyl
acetate and sodium acetate buffer. The product was analyzed by HPLC, UV, IR, and NMR.
The yield of genipin was 47.81% and its purity was over 98% (HPLC).
Keywords: immobilized β-glucosidase; geniposide; genipin; aqueous-organic two-phase
system
1. Introduction
Fructus Gardeniae is the dried ripe fruit of Gardenia jasminoides Ellis (Rubiaceae). It is widely
used in Traditional Chinese Medicine because of its cholagogue, sedative, diuretic, anti-inflammatory,
Molecules 2011,
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