Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines 英文参考文献.docVIP

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Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines 英文参考文献.doc

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Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines Simone D Castellarin1,2 and Gabriele Di Gaspero*1,2 Address: 1Istituto di Genomica Applicata, Parco Scientifico e Tecnologico Luigi Danieli, via Jacopo Linussio 51, 33100 Udine, Italy and 2Dipartimento di Scienze Agrarie e Ambientali, University of Udine, via delle Scienze 208, 33100 Udine, Italy Email: Simone D Castellarin - simone.castellarin@uniud.it; Gabriele Di Gaspero* - gabriele.digaspero@uniud.it * Corresponding author Published: 30 August 2007 Received: 30 March 2007 Accepted: 30 August 2007 BMC Plant Biology 2007, 7:46 doi:10.1186/1471-2229-7-46 This article is available from: /1471-2229/7/46 ? 2007 Castellarin and Di Gaspero; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Fruit coloration of red-skinned grapevines is mainly due to anthocyanin pigments. We analysed a panel of nine cultivars that included extreme phenotypes for berry colour, ranging from green (absence of anthocyanins) to red, purple, violet and blue. Expression of six genes of the anthocyanin pathway coding for flavanone-hydroxylase (F3H), flavonoid 3-hydroxylase (F3H), flavonoid 3,5-hydroxylase (F35H), UDP-glucose:flavonoid-3- O-glucosyltransferase (UFGT), glutathione-S-transferase (GST), O-methyltransferase (OMT) and four transcription factors (MybA, MybB, MybC, MybD) was analysed by quantitative RT-PCR at four developmental stages from before the onset of ripening until full maturity and compared to anthocyanin metabolites. Results: Total anthocyanin content at full maturity correlated well with the cu