Traceability of Plant Diet Contents in Raw Cow Milk Samples 英文参考文献.docVIP

Nutrients 2009, 1, 251-262; doi:10.3390/nu1020251 OPEN ACCESS nutrients ISSN 2072-6643 /journal/nutrients Article Traceability of Plant Diet Contents in Raw Cow Milk Samples Elena Ponzoni *, Francesco Mastromauro, Silvia Gianì and Diego Breviario Institute of Agricultural Biology and Biotechnology (IBBA), Italian National Research Council (CNR), Milan 20133, Italy; E-Mails: mastromauro@r.it (F.M.); giani@r.it (S.G.); terenzio@r.it (D.B.) * Author to whom correspondence should be addressed; E-Mail: ponzoni@r.it. Received: 9 October 2009 / Accepted: 1 December 2009 / Published: 4 December 2009 Abstract: The use of molecular marker in the dairy sector is gaining large acceptance as a reliable diagnostic approach for food authenticity and traceability. Using a PCR approach, the rbcL marker, a chloroplast-based gene, was selected to amplify plant DNA fragments in raw cow milk samples collected from stock farms or bought on the Italian market. rbcL-specific DNA fragments could be found in total milk, as well as in the skimmed and the cream fractions. When the PCR amplified fragments were sent to sequence, the nucleotide composition of the chromatogram reflected the multiple contents of the polyphytic diet. Keywords: DNA detection; traceability; feed 1. Introduction Requirements for traceability/product tracing have been introduced in national regulations as an effective means to contribute to product safety and consumer confidence. Under EU law, “traceability” means the ability to track any food, feed, food-producing animal or substance that will be used for consumption, through all stages of production, processing and distribution [1]. In particular the dairy sector has taken “the viewpoint that traceability/product tracing as a tool is not only relevant in the context of food inspection and certification systems, but may also be applied for industry-driven business reasons and