Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells 英文参考文献.docVIP

Available online /content/7/6/R1304 Research article Open Access Vol 7 No 6 Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells Farida Djouad1, Claire Bony1, Thomas H?upl2, Gilles Uzé3, Najiba Lahlou4, Pascale Louis-Plence1, Florence Apparailly1, Fran?ois Canovas5, Thierry Rème1, Jacques Sany5, Christian Jorgensen1 and Danièle No?l1 1INSERM Unit 475, Montpellier, France 2Rheumatology, Charité Hospital, Berlin, Germany 3CNRS, UMR 5124, Montpellier, France 4Hormonal Biology Laboratory, St Vincent de Paul Hospital, Paris, France 5Immuno-Rhumatologie, Lapeyronie Hospital, Montpellier, France Corresponding author: Danièle No?l, noel@montp.inserm.fr Received: 12 May 2005 Revisions requested: 18 Jul 2005 Revisions received: 26 Jul 2005 Accepted: 24 Aug 2005 Published: 20 Sep 2005 Arthritis Research Therapy 2005, 7:R1304-R1315 (DOI 10.1186/ar1827) This article is online at: /content/7/6/R1304 ? 2005 Djouad et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/ 2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Previous studies have reported that mesenchymal stem cells (MSC) may be isolated from the synovial membrane by the same protocol as that used for synovial fibroblast cultivation, suggesting that MSC correspond to a subset of the adherent cell population, as MSC from the stromal compartment of the bone marrow (BM). The aims of the present study were, first, to better characterize the MSC derived from the synovial membrane and, second, to compare systematically, in parallel, the MSC-containing cell populations isolated from BM and those derived from the synovium, using quantitative assays. Fluorescent-activated cell sorting analysis revealed that both populations were