Transposable elements in fish functional genomics technical challenges and perspectives 英文参考文献.docVIP

Review Transposable elements in fish functional genomics: technical challenges and perspectives Serguei Parinov and Alexander Emelyanov Temasek Life Sciences Laboratory, Research Link, The National University of Singapore, 117604, Singapore Correspondence: Serguei Parinov. E-mail: sergeypa@.sg Published: 31 October 2007 Genome Biology 2007, 8(Suppl 1):S6 (doi:10.1186/gb-2007-8-S1-S6) The electronic version of this article is the complete one and can be found online at /2007/8/S1/S6 ? 2007 BioMed Central Ltd Abstract The recent introduction of several transposable elements in zebrafish opens new frontiers for genetic manipulation in this important vertebrate model. This review discusses transposable elements as mutagenesis tools for fish functional genomics. We review various mutagenesis strategies that were previously applied in other genetic models, such as Drosophila, Arabidopsis, and mouse, that may be beneficial if applied in fish. We also discuss the forthcoming challenges of high-throughput functional genomics in fish. Introduction The choice of many transposon vectors provides zebrafish biologists with a plethora of possibilities, but it also raises the issue of which to use and when. The Tc3 element from Caenorrhabditis elegans and the mariner (Mos1) element from Drosophila mauritiana were the first transposon vectors used to generate transgenic fish (Table 1) [1,2]. However, since the initial reports, no further developments using these transposons have been described in the litera- ture. Two transposable elements of fish origin, Tol2 from medaka (a member of the hAT [hobo/Ac/Tam3] super- family) and Sleeping Beauty (a synthetic member of the Tc1/mariner superfamily), have both become widely used in the past few years (Table 1) [7-12]. Recently, a transposable system from plants has been added to this arsenal. The modifi