Transcriptomic analysis of grape (Vitis vinifera L.) leaves during and after recovery from heat stress 英文参考文献.docVIP

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Transcriptomic analysis of grape (Vitis vinifera L.) leaves during and after recovery from heat stress 英文参考文献.doc

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Transcriptomic analysis of grape (Vitis vinifera L.) leaves during and after recovery from heat stress 英文参考文献

Liuetal.BMCPlantBiology2012,12:174 /1471-2229/12/174 RESEARCH ARTICLE OpenAccess Transcriptomicanalysisofgrape(VitisviniferaL.) leavesduringandafterrecoveryfromheatstress Guo-TianLiu1,2,Jun-FangWang1,2,GrantCramer3,Zhan-WuDai4,WeiDuan1,Hong-GuoXu1,Ben-HongWu1, Pei-GeFan1,Li-JunWang1*andShao-HuaLi1,5* Abstract Background:Grapesareamajorfruitcroparoundtheworld.Heatstresscansignificantlyreducegrapeyieldand quality.Changesatthemolecularlevelinresponsetoheatstressandsubsequentrecoveryarepoorlyunderstood. Toelucidatetheeffectofheatstressandsubsequentrecoveryonexpressionofgenesbygrapeleavesrepresenting theclassicheatstressresponseandthermotolerancemechanisms,transcriptabundanceofgrape(VitisviniferaL.) leaveswasquantifiedusingtheAffymetrixGrapeGenomeoligonucleotidemicroarray(15,700transcripts),followed byquantitativeReal-TimePCRvalidationforsometranscriptprofiles. Results:Wefoundthatabout8%ofthetotalprobesetswereresponsivetoheatstressand/ortosubsequent recoveryingrapeleaves.Theheatstressandrecoveryresponseswerecharacterizedbydifferenttranscriptional changes.Thenumberofheatstress-regulatedgeneswasalmosttwicethenumberofrecovery-regulatedgenes. Theresponsivegenesidentifiedinthisstudybelongtoalargenumberofimportanttraitsandbiologicalpathways, includingcellrescue(i.e.,antioxidantenzymes),proteinfate(i.e.,HSPs),primaryandsecondarymetabolism, transcriptionfactors,signaltransduction,anddevelopment.Wehaveidentifiedsomecommongenesandheat shockfactors(HSFs)thatweremodulateddifferentiallybyheatstressandrecovery.MostHSPgeneswere upregulatedbyheatstressbutweredownregulatedbytherecovery.Ontheotherhand,somespecificHSPgenes orHSFswereuniquelyresponsivetoheatstressorrecovery. Conclusion:Theeffectofheatstressandrecoveryongrapeappearstobeassociatedwithmultipleprocessesand mechanismsincludingstress-relatedgenes,transcriptionfactors,andmetabolism.Heatstressandrecoveryelicited commonup-ordownregulatedgenesaswellasuniquesetsofresponsivegenes.Moreover,somegeneswere regulatedinoppositedirectionsb