Two New Cyanogenic Glucosides from the Leaves of Hydrangea macrophylla 英文参考文献.docVIP

Molecules 2012, 17, 5396-5403; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Two New Cyanogenic Glucosides from the Leaves of Hydrangea macrophylla Chun-Juan Yang 1, Zhi-Bin Wang 2, Da-Ling Zhu 1, Ying Yu 2, Yin-Ting Lei 2 and Yan Liu 3,* 1 College of Pharmacy, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin 150081, China; E-Mails: chunjuanyang@126.com (C.-J.Y.); dalingz@ (D.-L.Z.) 2 Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150040, China; E-Mails: wzbmailbox@126.com (Z.-B.W.); 763775361@ (Y.Y.); yintinglei@ (Y.-T.L.) 3 Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China * Author to whom correspondence should be addressed; E-Mail: liuyanhlj@163.com; Tel.: +86-451-8660-5765; Fax: +86-451-8661-4073. Received: 27 March 2012; in revised form: 22 April 2012 / Accepted: 27 April 2012 / Published: 8 May 2012 Abstract: Chemical investigation of the ethanol extract of the aerial parts of Hydrangea macrophylla collected in the Sichuan Province of China resulted in the isolation of two new cyanogenic glucosides. Their structures were elucidated as [(2R)-2- (?-D-glucopyranosyloxy)-2-(3,4-dimethoxy-phenyl)] acetonitrile (1) and {(2R)-2-[?-D- glucopyranosyl(1?6)?-D-glucopyranosyloxy]-2-(3-hydroxy-4-methoxy-phenyl)}acetonitrile (2) on the basis of extensive spectroscopic analysis (1D, 2D NMR and HRESIMS) and chemical studies. Keywords: Hydrangea macrophylla; Saxifragaceae; cyanogenic glucoside 1. Introduction Hydrangea macrophylla (Thunb.) Ser., a Saxifragaceae plant, is widely cultivated in many countries, including China and Japan. Known biologically active components from the genus Hydrangea are dihydroisocoumarins and their glycosides [1–3], iridoid glycosides, secoiri