Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.docVIP

/2002/3/7/research/0034.1 Research Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Jo Vandesompele, Katleen De Preter, ilip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe and rank Speleman Address: Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B-9000 Ghent, Belgium. Correspondence: rank Speleman. E-mail: franki.speleman@rug.ac.be Published: 18 June 2002 Received: 20 December 2001 Revised: 10 April 2002 Accepted: 7 May 2002 GenomeBiology 2002, 3(7):research0034.1–0034.11 The electron